Figures & data
Figure 1 GBM tissues exhibited altered expression of circPTK2 and mature miR-23a but not premature miR-23a. Total RNAs were isolated from paired cancer and non-cancer tissues of 60 GBM patients, and the expression of circPTK2 (A), mature miR-23a (B), and premature miR-23a (C) were analyzed using RT-qPCR. HemI 1.0 software was used to plot heatmaps to represent differential gene expression.
Figure 2 A significant and inverse correlation was observed between circPTK2 and mature miR-23a (A) but not between circPTK2 and premature miR-23a (B).
Figure 3 The correlations between circPTK2 and mature miR-23a or premature miR-23a across GBM tissues were analyzed by Pearson’s correlation coefficient. LN-229 and U-138 cells were transfected with circPTK2 expression vector or miR-23a mimic, and the overexpression was checked every 24h until 96h (A). The effects of circPTK2 expression vector transfection on mature miR-23a (B) and premature miR-23a (C) and the effects of miR-23a mimic transfection on the expression of circPTK2 (D) at each time point were also analyzed by RT-qPCR. *p<0.05.
Figure 4 CircPTK2 overexpression suppressed GBM cell invasion and migration via miR-23a. Transwell assay was performed to analyze the role of circPTK2 (A) and miR-23a (B) in regulating the invasion and migration of LN-229 and U-138 cells. *p<0.05.
Availability of Supporting Data
The data that support the findings of this study are not publicly available due to their containing information that could compromise the privacy of research participants but available on request from the corresponding author: Minxue Lian, Department of Neurosurgery, First Affiliated Hospital of Xi’an Jiaotong University, No. 277 West Yanta Road, Xi’an City, Shaanxi Province, 710061, P. R. China. E-mail:[email protected].