The Interaction of LAMA2 and Duration of Illness Affects the Thickness of the Right Transverse Temporal Gyrus in Major Depressive Disorder

Figures & data

Table 1 Demographic and Clinical Assessments of Participants

	MDD (n=89)	HC (n=60)	p
Age, years	45.28±13.28	39.73±13.54	0.014^b
Sex, male/female, n	39/50	29/31	0.588^a
Education, years	10.70±4.12	11.84±4.56	0.062^b
DOI, months	59.69±86.73
Age of onset, years	40.72±14.49
Episodes, n	2.11±2.07
HAMD-17 score (0w)	22.34±3.64
rs2229848 (CC/CT/TT)	11/45/33	19/25/16	0.015^a
rs2229849 (CC/GC/GG)	33/46/10	16/26/18	0.015^a
rs2229850 (AA/GA/GG)	10/46/33	19/27/14	0.006^a
rs2275214 (CC/GC/GG)	47/32/10	15/28/17	0.001^a
rs2784895 (CC/CT/TT)	9/33/47	11/23/17	0.011^a
rs6569604 (CC/GC/GG)	15/38/36	23/23/14	0.008^a
rs6938825 (AA/CA/CC)	32/44/13	15/26/19	0.039^a

Note: ^aChi-squared test; ^bIndependent sample t-test.

Abbreviations: MDD, major depressive disorder, HC, healthy controls; DOI, duration of illness, HAMD-17, 17-item Hamilton Depression Rating Scale.

Figure 1 Differences in thickness of right transverse temporal gyrus and right parahippocampal gyrus between HC and MDD. (a) Thickness of right parahippocampal gyrus in the MDD was significantly lower than HC (p < 0.001). (b) Thickness of right transverse temporal gyrus in the MDD was significantly lower than HC (p < 0.001). ***p < 0.001.

Abbreviations: TH, cortical thickness; HC, health control; MDD, major depressive disorder.

Figure 2 Scatter-box violin plot showing the difference of thickness in the right transverse temporal gyrus and right parahippocampal gyrus in MDD patients with different DOI. (a) Thickness of right transverse temporal gyrus in MDD patients with DOI ≤ 1 year was significantly lower than that in the group > 1 year (p = 0.016). (b) There was no significant difference in thickness of right parahippocampal gyrus between MDD patients with DOI ≤ 1 year and >1 year (p = 0.231).

Abbreviations: TH, cortical thickness; DOI, duration of illness.

Table 2 Interaction of LAMA2 SNPs-Diagnosis on Thickness of Right Transverse Temporal Gyrus and Right Parahippocampal Gyrus

	SNP	Right Transverse Temporal		Right Parahippocampal
		F	Correct p-value	F	Correct p-value
Gene×Diagnosis	rs6569604	3.170	0.07	3.781	0.011
	rs2784895	3.008	0.046	2.665	0.035
	rs2229850	2.855	0.04	2.588	0.028
	rs2229849	2.847	0.032	2.589	0.033
	rs2229848	2.809	0.027	2.822	0.032
	rs6938825	2.201	0.067	2.867	0.04
	rs2275214	2.068	0.074	4.317	0.007

Note: All p-value are FDR corrected (p < 0.05).

Abbreviation: SNP, single nucleotide polymorphisms.

Table 3 Interaction of LAMA2 SNPs-DOI on Thickness of Right Transverse Temporal Gyrus and Right Parahippocampal Gyrus

	SNP	Right Transverse Temporal		Right Parahippocampal
		F	Correct p-value	F	Correct p-value
Gene×DOI	rs6569604	4.247	0.014	1.702	0.200
	rs2784895	2.986	0.022	3.238	0.070
	rs2229850	3.062	0.033	2.514	0.126
	rs2229849	3.062	0.025	2.514	0.084
	rs2229848	3.102	0.046	1.806	0.212
	rs6938825	2.342	0.056	1.285	0.278
	rs2275214	1.625	0.163	1.340	0.299

Note: All p-value are FDR corrected (p < 0.05).

Abbreviations: SNP, single nucleotide polymorphisms; DOI, duration of illness.

Figure 3 (a–e) The interaction effect of the five SNP of LAMA2 and MDD on thickness of right transverse temporal gyrus. rs2229850, rs2229848, rs2229849, rs6569604 and rs2784895 had significant interaction with DOI on thickness of right transverse temporal gyrus, but the minor allele of rs2229850 shows opposite effects to the other four SNPs on thickness of right transverse temporal gyrus (p < 0.05).

Abbreviation: DOI, duration of illness.