Possible association between haplotypes of the FKBP5 gene and suicidal bipolar disorder, but not with melancholic depression and psychotic features, in the course of bipolar disorder

Figures & data

Table 1 FKBP5 gene polymorphism description

SNP	Substitution	Location	Method	Assay ID
rs1360780	C/T	Intron 9	TaqMan	C___8852038_10
rs755658	C/T	Intron 3	TaqMan	C___594063_20
rs9470080	C/T	Intron 10	TaqMan	C_____92160_10
rs4713916	A/G	Intron 10	TaqMan	C___1979246_10
rs7748266	C/T	Intron 8	TaqMan	C__29802748_10
rs9296158	A/G	Intron 6	TaqMan	C____1256775_10
rs9394309	A/G	Intron 10	TaqMan	C____1256794_20
rs3800373	A/C	3′ UTR	TaqMan	C__27489960_10

Abbreviations: SNP, single-nucleotide polymorphism; FKBP5, FK506 binding protein 5; UTR, untranslated region.

Table 2 Check markers for subtypes of BP for FKBP5 gene

Number	SNP	Pos chromos	ObsHET	PredHET	HWE	% zgenotyp	MAF	Alleles
Check markers melancholic
1	rs3800373	35650454	0.366	0.366	1.0	99.2	0.241	A:C
2	rs755658	35657648	0.168	0.168	1.0	95.4	0.092	C:T
3	rs9296158	35675060	0.365	0.373	0.5832	98.3	0.248	G:A
4	rs7748266	35700722	0.213	0.226	0.1241	97.3	0.13	C:T
5	rs1360780	35715549	0.365	0.374	0.4929	97.4	0.249	C:T
6	rs9394309	35729759	0.376	0.387	0.4897	96.4	0.262	A:G
7	rs9470080	35754413	0.399	0.4	0.9398	98.9	0.277	C:T
8	rs4713916	35777961	0.37	0.379	0.5371	98.2	0.254	G:A
Check markers psychotic
1	rs3800373	35650454	0.367	0.37	0.879	98.9	0.245	A:C
2	rs755658	35657648	0.168	0.167	1.0	95.4	0.092	C:T
3	rs9296158	35675060	0.364	0.377	0.3189	98.3	0.252	G:A
4	rs7748266	35700722	0.222	0.24	0.0375	97.1	0.139	C:T
5	rs1360780	35715549	0.369	0.38	0.4012	97.2	0.255	C:T
6	rs9394309	35729759	0.391	0.396	0.7577	96.2	0.272	A:G
7	rs9470080	35754413	0.408	0.408	1.0	98.7	0.285	C:T
8	rs4713916	35777961	0.393	0.387	0.7322	98.1	0.262	G:A
Check markers suicide
1	rs3800373	35650454	0.365	0.36	0.7891	98.9	0.235	A:C
2	rs755658	35657648	0.166	0.164	0.9119	95.6	0.09	C:T
3	rs9296158	35675060	0.363	0.369	0.635	98.4	0.244	G:A
4	rs7748266	35700722	0.208	0.223	0.0643	97.2	0.128	C:T
5	rs1360780	35715549	0.365	0.373	0.5708	97.3	0.248	C:T
6	rs9394309	35729759	0.377	0.385	0.5996	96.8	0.26	A:G
7	rs9470080	35754413	0.401	0.399	0.9346	99.0	0.275	C:T
8	rs4713916	35777961	0.372	0.376	0.8302	98.3	0.251	G:A

Abbreviations: FKBP5, FK506 binding protein 5; SNP, single-nucleotide polymorphism; BP, bipolar disorder; ObsHET, observed heterozygous; PredHET, predicted heterozygous; MAF, minor allele frequency; HWE, Hardy–Weinberg equilibrium; Pos chromos, chromosomal position; zgenotyp, % genotyped (=success rate or call rate).

Figure 1 Relative positions and LD estimates between FKBP5 polymorphisms in the analyzed population-patients with melancholic depression.

Note: Colored squares correspond to D’ values with numerical estimates given within the squares.

Figure 2 Relative positions and LD estimates between FKBP5 polymorphisms in the analyzed population-patients with psychotic bipolar disorder.

Note: Colored squares correspond to D’ values with numerical estimates given within the squares.

Figure 3 Relative positions and LD estimates between FKBP5 polymorphisms in the analyzed population-bipolar patients with suicide attempts.

Note: Colored squares correspond to D’ values with numerical estimates given within the squares.

Table 3 Haplotypes of FKBP5 gene and subtypes of BP disorder

Haplotype	Freq	Case: control frequencies	Chi-square	P-value
Association-haplotypes melancholic
ACGCCAC	0.718	0.719, 0.718	0.001	0.9809
CCATTGT	0.117	0.094, 0.121	1.484	0.2232
CTACTGT	0.087	0.100, 0.085	0.624	0.4294
ACGCCGT	0.029	0.031, 0.029	0.038	0.8449
CCACTAT	0.016	0.027, 0.014	2.263	0.1325
CCACTGT	0.012	0.020, 0.011	1.466	0.2261
Association-haplotypes psychotic
ACGCCAC	0.710	0.679, 0.718	2.193	0.1387
CCATTGT	0.122	0.130, 0.121	0.24	0.6239
CTACTGT	0.087	0.095, 0.085	0.383	0.5362
ACGCCGT	0.031	0.038, 0.029	0.926	0.336
CCACTAT	0.016	0.022, 0.014	1.156	0.2822
Association-haplotypes suicide
ACGCCAC	0.721	0.736, 0.718	0.429	0.5124
CCATTGT	0.112	0.071, 0.121	6.261	0.0123*
CTACTGT	0.085	0.083, 0.085	0.019	0.8906
ACGCCGT	0.029	0.030, 0.029	0.009	0.9265
CCACTAT	0.018	0.033, 0.014	5.13	0.0235*
CCACTGT	0.013	0.023, 0.011	2.906	0.0883
ACATTGT	0.010	0.016, 0.009	1.166	0.2802

Note:

* Note: Indicates association.

Abbreviations: FKBP5, FK506 binding protein 5; BP, bipolar disorder; Freq, frequency.

Table 4 Alleles for FKBP5 gene and subtypes of BP disorder

Lp	SNP	Assoc allele	Case: control freq	Chi-square	P-value
Association-alleles melancholic
1	rs3800373	C	0.244, 0.240	0.017	0.897
2	rs755658	T	0.100, 0.091	0.201	0.6543
3	rs9296158	A	0.250, 0.247	0.008	0.9267
4	rs7748266	C	0.898, 0.865	2.177	0.1401
5	rs1360780	C	0.754, 0.750	0.018	0.8921
6	rs9394309	A	0.760, 0.734	0.727	0.3939
7	rs9470080	T	0.278, 0.277	0.001	0.9744
8	rs4713916	G	0.766, 0.743	0.603	0.4373
Association-alleles psychotic
1	rs3800373	C	0.262, 0.240	0.764	0.382
2	rs755658	T	0.094, 0.091	0.036	0.8504
3	rs9296158	A	0.271, 0.247	0.896	0.3439
4	rs7748266	T	0.155, 0.135	0.96	0.3271
5	rs1360780	T	0.276, 0.250	1.097	0.295
6	rs9394309	G	0.294, 0.266	1.182	0.2769
7	rs9470080	T	0.319, 0.277	2.627	0.1051
8	rs4713916	A	0.282, 0.257	1.002	0.3168
Association-alleles suicide
1	rs3800373	A	0.790, 0.760	1.203	0.2728
2	rs755658	C	0.916, 0.909	0.156	0.6924
3	rs9296158	G	0.774, 0.753	0.5 85	0.4443
4	rs7748266	C	0.907, 0.865	3.905	0.0481*
5	rs1360780	C	0.764, 0.750	0.245	0.6208
6	rs9394309	A	0.767, 0.734	1.35	0.2453
7	rs9470080	C	0.733, 0.723	0.128	0.7202
8	rs4713916	G	0.780, 0.743	1.831	0.176

Note:

* Indicates association.

Abbreviations: FKBP5, FK506 binding protein 5; SNP, single-nucleotide polymorphism; BP, bipolar disorder; Assoc, associated; freq, frequency.