Figures & data
Notes: (A) Mg-free artificial cerebrospinal fluid plus 4-aminopyridine (4-AP) induced epileptiform discharges in hippocampal CA1 pyramidal neurons. (B) LPS facilitated epileptiform activity; the facilitation remained 30 minutes after LPS washout. (C) Representation of the epileptiform discharges induced by Mg-free artificial cerebrospinal fluid plus 4-AP (Left) and 30 minutes after 10 μg/mL LPS added (Right). (D) Summary data (n=8) showed that LPS added after 30 minutes increased the burst counts in 5 minutes recording. (E) Expanded burst marked with black squares in (C). Notably, both frequency of burst and number of spikes per burst were increased after LPS application. (F) Summary data (n=8) showed mean counts of spikes per burst recorded in the control condition and 30 minutes after 10 μg/mL LPS was added. ***Significant difference (P<0.001; paired-sample Student’s t-test). Current clamp recordings were performed in hippocampal CA1 pyramidal neurons. Holding potential was −70 mV.
Notes: Representative traces of eEPSC (A) and eIPSC (B) recorded in artificial cerebrospinal fluid (black lines) and 30 minutes after lipopolysaccharide was added (gray lines) under voltage clamp. Summary data depicted mean eEPSC (C) and eIPSC (D) peak amplitudes in control conditions and in the presence of lipopolysaccharide (n=5). **Significant difference (P<0.01; paired-sample Student’s t-test). Holding potential was −70 mV for recording eEPSCs and −40 mV for eIPSCs.
Abbreviation: ms, millisecond.
Abbreviation: ms, millisecond.