Lisdexamfetamine prodrug activation by peptidase-mediated hydrolysis in the cytosol of red blood cells

Figures & data

Figure 1 LDX bioconversion to d-amphetamine in blood and blood cell extracts.

Notes: Data for d-amphetamine are presented as the mean of four replicates (whole blood and negative control) or two replicates (other samples), with error bars showing SEM. Points without visible error bars have SEMs smaller than the height of the data point marker. Values for intact LDX remaining at 4 hours are expressed as percentages of the mean LDX concentration at 0 hours.
Abbreviations: LDX, lisdexamfetamine dimesylate; SEM, standard error of the mean.

Figure 2 Effect of protease inhibitor cocktail and BNPP on d-amphetamine production from LDX in whole blood or cytosolic extract.

Notes: Data for d-amphetamine are presented as the mean of two replicates, with error bars showing SEM. Points without visible error bars have SEMs smaller than the height of the data point marker. Values for intact LDX remaining at 4 hours are expressed as percentages of the mean LDX concentration at 0 hours.
Abbreviations: BNPP, bis(4-nitrophenyl)phosphate; LDX, lisdexamfetamine dimesylate; SEM, standard error of the mean.

Figure 3 Effect of individual components of protease inhibitor cocktail on d-amphetamine production from LDX in red blood cell cytosolic extract.

Notes: Data for d-amphetamine are presented as the mean of two replicates, with error bars showing SEM. Points without visible error bars have SEMs smaller than the height of the data point marker. Values for intact LDX remaining at 4 hours are expressed as percentages of the mean LDX concentration at 0 hours.
Abbreviations: AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride; EDTA, ethylenediaminetetra-acetic acid; LDX, lisdexamfetamine dimesylate; SEM, standard error of the mean.