Hypnotic effect of the essential oil from the leaves of Myrtus communis on mice

Figures & data

Figure 1 Effect of EO from leaves of Myrtus communis.

Notes: (A) Effect of EO from leaves of Myrtus communis on sleep latency in PIST test. (B) Effect of the EO from leaves of M. communis on sleep duration in PIST test. The doses of EO used were 500, 600, 800, and 1,000 mg/kg (po). The standard used was diazepam (3 mg/kg, po). n=6 in each group. Values represent mean ± SEM. P<0.05 between the test groups and control, and P<0.01 between standard and control groups (one-way ANOVA followed by Dunnet’s test). Grp represents group administered with the corresponding doses (mg/kg).
Abbreviations: EO, essential oil; PIST, pentobarbital-induced sleeping time; po, orally; SEM, standard error of the mean; ANOVA, analysis of variance; Std, standard.

Figure 2 Effect of the EO on the negative response in chimney test.

Notes: Standard group was administered diazepam (3 mg/kg). Values represent percentage of mice showing negative effects in the tests ± SEM. n=6. P<0.01 between Groups III–VI and Group I, and P<0.01 between Group I and Group II. (Control used were administered 0.4 mL of 5% Tween 80, and standard were administered 1 mg/kg of diazepam. Values represent percentages of mice (n=6) showing negative effects in the chimney tests 1 hour after treatment with the vehicle and EO of varying doses, and 30 minutes after treatment with the standard, diazepam [1 mg/kg, po]). Grp represents group administered with the corresponding doses (mg/kg).
Abbreviations: EO, essential oil; SEM, standard error of the mean; po, orally.

Figure 3 Effect of the EO on the negative response in traction test.

Notes: Standard group was administered diazepam (3 mg/kg). Values represent mean percentage of mice showing negative effects in the tests ± SEM. P<0.05 between Groups III–VI and Group I, and P<0.01 between Group I and Group II. (n=6. Values represent percentages of mice (n=6) showing negative effects in the traction tests 1 hour after treatment with the vehicle and EO of varying doses, and 30 minutes after treatment with diazepam. P<0.05 between the test groups and control, and P<0.01 between diazepam and control.) Grp represents group administered with the corresponding doses (mg/kg).
Abbreviations: EO, essential oil; SEM, standard error of the mean.