Fluoroquinolone antibacterial eye drops: effects on normal human corneal epithelium, stroma, and endothelium

Figures & data

Table 1 The observation items and details of each item

Observation item	Timing of observation		Observation of cornea			Endothelium
	2 weeks before study entry	After 7-days of instillation	Epithelium	Stroma	Evaluation
Informed consent	○
Subject background	○
Concomitant drugs	○	○
Visual acuity test	○	○
Treatment compliance		○
Slit-lamp microscopy	○	○	✓			Observation of the corneal epithelium
Anterior-segment pictures (fluorescein staining) (BUT)	○	○	✓			Evaluation of the influence on the corneal epithelium
Specular microscope	○	○			✓	Observation of the corneal endothelium. Measurement of endothelial cell density, and coefficient of variation (CV)
DR-1 (BUT)	○	○	✓			Evaluation of influence on the corneal epithelium based on tear film Iipid layer grading
Schirmer l test (BUT)	○	○	✓			Lacrimation function based on tear volume
Pentacarn (Corneal thickness)	○	○		✓		Measurement of corneal thickness (influence on the corneal endothelium)
HRT II-RCM	○	○		✓		Morphological observation of the corneal stroma Evaluation of cell density
Adverse events	○	○
Questionnaire to assess satisfaction with use		○				Assessment of irritating sensation and drop comfort upon instillation using a VAS

Figure 1 Corneal epithelial defects according to AD grading. After 7 days of instillation, corneal epithelial defects were observed in eight eyes of the subjects receiving MFLX and seven eyes of the subjects receiving LVFX. AD grading of the 15 eyes was A1D1 for 14 eyes and A2D1 for the remaining eye. No statistically significant difference between the groups was observed in the incidence of epithelial defects (P = 0.675, t-test).