Epiretinal membrane in a subject after transvitreal delivery of palucorcel (CNTO 2476)

Figures & data

Figure 1 Appearance of subretinal bleb containing palucorcel injection immediately after completion of the injection of the cellular product at the time of surgery.

Notes: Note the small air bubble at the opening of the retinotomy site. Short, black arrows demarcate the edge of the subretinal bleb of injected palucorcel.

Figure 2 Fundus photographs of the subject’s eye (A, B) before the procedure and (C, D) at 5 weeks after surgery.^a

Note: ^aThe area of retinal detachment can be seen superior to the optic disc.

Figure 3 Epiretinal membrane immunocytochemistry analysis using (A, B) a combination of anti-vimentin (green), anti-neurofilament (red), ricin (blue), and Hoescht (white) and (C, D) a combination of anti-vimentin (green), anti-ezrin (red), ricin (blue), and Hoescht (white).

Notes: Figures (B and D) show the four channels separately for Figures (A and C), respectively. Mag Bars, (A, B): 50 microns; (C, D): 100 microns.^{a,b a}Anti-vimentin was used to identify Müller cells (but could also show donor cells); anti-neurofilament was used to identify ganglion cell neurites; ricin was used to identify microglia and macrophages; anti-ezrin was used to identify retinal pigment epithelial cells (red arrows in part C); Hoescht was used to identify nuclei. ^bFigures are projections of Z-stacks comprising 6 to 10 images taken at 1-micron intervals.

Figure 4 Epiretinal membrane immunocytochemistry analysis using (A, B) a combination of anti-vimentin (green), anti-GFAP (red), ricin (blue), and Hoescht (white) and (C, D) a combination of anti-vimentin (green), anti-Mib1 (red), ricin (blue), and Hoescht (white).

Notes: Figures (B and D) show the four channels separately for (A and C), respectively. Mag bars: (A, B): 100 microns; (C, D): 500 microns.^{a,b a}Anti-vimentin was used to identify Müller cells (but could also show donor cells); anti-GFAP was used to identify glial cells; ricin was used to identify microglia and macrophages; Hoescht was used to identify nuclei; and anti-Mib1 was used to identify dividing cells. ^bFigures are projections of Z-stacks comprising 6 to 10 images taken at 1-micron intervals.

Figure 5 Fluorescence in situ hybridization staining of the epiretinal membrane using (A) an X (green arrows) chromosome probe and (B) X (green arrows) and Y (red arrows) chromosome probes.^a

Note: ^aThe nuclei in part B are positive for both the X (green arrows) and Y (red arrows) chromosome probes.