The development of a reference database with the Topcon 3D OCT-1 Maestro

Figures & data

Figure 1 Measurement areas for macula Early Treatment Diabetic Retinopathy Study grid (A), macula 6 circle (B), and circumpapillary RNFL circle (C) overlaid with example projected images. Single-frame OCT B-scan images overlaid with boundaries (green and blue lines) demonstrated the retinal layers in various retinal thickness measurements, including full retinal thickness (D); ganglion cell + inner plexiform layers (distance between the 2 green boundaries), ganglion cell complex (distance between the blue and green boundaries, vitreal to inner nuclear layer) (E); and retinal neural fiber layer (F). All the images were set with right eye orientation.

Abbreviations: F, foveal; I, inferior; N, nasal; OCT, optical coherence tomography; RNFL, retinal nerve fiber layer; S, superior; T, temporal.

Figure 2 Quantile regression fits of various macula measurements from 12×9 wide and 6×6 macula scans are shown.

Notes: Age did not have a significant effect on FRT near the foveal center (top row). There were negative associations of age with macula GCL + IPL thickness and GCC thickness (middle and bottom rows). The 1st (red line), 5th (green line), 50th (yellow line), 95th (blue line), and 99th (cyan line) percentiles were estimated by non-crossing quantile regressions based on the data displayed in the scatter plots.
Abbreviations: FRT, full retinal thickness; GCC, ganglion cell complex; GCL, ganglion cell; IPL, inner plexiform layer.

Table 1 Summary of selected scan parameters

Parameter	Scan size 12 ×9 mm				Scan size 6× 6 mm
	N	Mean (SD)	95% CI	Median	N	Mean (SD)	95% CI	Median
Central fovea FRT (μm)	395	237.079 (20.899)	235.012–239.146	235.520	395	234.000 (20.657)	231.956–236.043	232.290
Average GCL + IPL (μm)	398	71.363 (5.924)	70.779–71.946	71.450	397	71.726 (5.880)	71.146–72.306	71.700
Average GCC (μm)	398	105.949 (8.533)	105.108–106.789	105.850	397	106.268 (8.602)	105.419–107.116	105.800
Average RNFL (μm)	398	104.720 (11.829)	103.555–105.886	105.225	398	104.036 (11.341)	102.918–105.153	103.990
C/D vertical	398	0.489 (0.200)	0.469–0.508	0.520	398	0.480 (0.190)	0.462–0.499	0.510
C/D area (mm^2)	398	0.278 (0.179)	0.261–0.296	0.260	398	0.280 (0.169)	0.263–0.297	0.270
Disc area (mm^2)	398	2.264 (0.409)	2.223–2.304	2.230	398	2.102 (0.414)	2.061–2.142	2.065
Cup area (mm^2)	398	0.655 (0.485)	0.608–0.703	0.570	398	0.613 (0.432)	0.570–0.655	0.545
Rim area (mm^2)	398	1.608 (0.415)	1.568–1.649	1.580	398	1.489 (0.383)	1.452–1.527	1.460
Cup volume (mm^3)	398	0.127 (0.143)	0.112–0.141	0.080	398	0.125 (0.135)	0.112–0.139	0.080
Rim volume (mm^3)	398	0.287 (0.153)	0.272–0.302	0.260	398	0.264 (0.140)	0.250–0.278	0.240

Note: 95% CI=95% CI for mean based on t-distribution.

Abbreviations: C/D, cup/disc; CL, ganglion cell; FRT, full retinal thickness; GCC, ganglion cell complex; GCL, ganglion cell; IPL, inner plexiform layer; RNFL, retinal nerve fiber layer.

Figure 3 The relationships of cpRNFL thickness with age and disc area displayed in the 3D scatter plots (A). The insets show the effect of age and disc area on cpRNFL thickness based on median fits (50th percentile). The cpRNFL circle profile reference limits are shown (B).

Note: The green region, considered within normal limits, encompassed measurements between the 95th and the 5th percentile of the reference data; the yellow region consisted of measurements between the 5th and 1st percentiles of the reference data; the red region consisted of measurements <1% of the reference data.
Abbreviation: cpRNFL, circumpapillary retinal nerve fiber layer.