Influence of bacterial burden on meibomian gland dysfunction and ocular surface disease

Figures & data

Figure 1 Increasing Ocular Surface Disease Index (OSDI) scores by group (ANOVA; p<0.001).

Figure 2 Increasing meibomian gland loss in right lower lid (RLL) by group (ANOVA; p=0.005).

Figure 3 Increasing meibomian gland loss in left upper lid (LUL) by group (ANOVA; p=0.026).

Figure 4 Increasing meibomian gland loss in left lower lid (LLL) by group (ANOVA, p=0.032).

Figure 5 Increasing conjunctival staining across groups, right eye (ANOVA; p=0.004).

Figure 6 Increasing conjunctival staining across groups, left eye (ANOVA; p=0.041).

Table 1 Group A results

Parameter	OD	OS
TOT	302.6±6.5	304.9±18.58
%MMP-9+	10	40
Schirmer 1 score	22.7±9.9	20.8±9.6
Conjunctival LG score	0.85±0.78	0.8±0.93
Corneal LG score	0.25±0.6	0.35±0.63
MG expression score^a	2.3±1.27^a	2.25±1.17^a
% Total positive cultures	50	60
% Positive lid margin cultures^b	90%	100%
% Positive MG cultures^b	100%	66%
% Positive CNS^b	100	100
%Erythromycin resistance^b	0	33
%Tetracycline resistance^b	0	0

Notes: ^aStatistically significant results for MG Expression score. ^bPercentage of culture positive group.

Abbreviations: MG, meibomian gland; OD, right eye; OS, left eye; LG, lissamine green.

Table 2 Group B results

Parameter	OD	OS
TOT	303.3±18.4	298.9±11.04
%MMP-9+	40	40
Schirmer 1 score	15.8±10.8	14.9±9.3
Conjunctival LG score	1.05±0.47	1.1±0.4
Corneal LG score	0.5±0.55	0.6±0.58
MG expression score	3.3±1.04	3.4±0.92
%Total positive cultures	50	50
% Positive lid margin cultures^a	80	80
% Positive MG cultures^a	80	40
% Positive CNS^a	100	80
%Erythromycin resistance^a	0	30
%Tetracycline resistance^a	0	10

Note: ^aPercentage of culture positive group.

Abbreviations: MG, meibomian gland; OD, right eye; OS, left eye; LG, lissamine green.

Table 3 Group C results

Parameter	OD	OS
TOT	300.6±15.03	299.2±15.6
%MMP-9+	50	60
Schirmer 1 score	15.1±5.6	17.9±6.33
Conjunctival LG score	0.9±0.5	1.2±0.9
Corneal LG score	0.45±0.35	0.4±0.4
MG expression score	3.2±0.7	3.2±0.7
% Total positive cultures	40	70
% Positive lid margin cultures^a	75	86
% Positive MG cultures^a	50	43
% Positive CNS^a	75	71
%Erythromycin resistance^a	10	30
%Tetracycline resistance^a	0	10

Notes: ^aPercentage of culture positive group.

Abbreviations: MG, meibomian gland; OD, right eye; OS, left eye; LG, lissamine green.

Table 4 Group D results

Parameter	OD	OS
TOT	301.7±11.4	301.6±13.76
%MMP-9+	50	60
Schirmer 1 score	12.5±8.5	12.8±8.81
Conjunctival LG score^a	2±0.95^a	2.05±1.9^a
Corneal LG score	0.4±0.2	0.45±0.27
MG expression score	3.3±1.1	3.2±1.17
% Total positive cultures	40	30
% Positive lid margin cultures^b	50	100
% Positive MG cultures^b	50	66
% Positive CNS^b	25	66
%Erythromycin resistance^b	0	20
%Tetracycline resistance^b	0	0

Notes: ^aStatistically significant results for conjunctival lissamine stain score. ^bPercentage of culture positive group.

Abbreviations: MG, meibomian gland; OD, right eye; OS, left eye; LG, lissamine green.

Table 5 Percentage meibomian gland loss across groups

%MG loss	RUL	RLL	LUL	LLL
Group A	21±15.13^a	28.5±17.5	18±12.3^a	32±18.2
Group B	36.5±20.9	32±17.9	35.5±20.2	37±15.03
Group C	29.5±10.9	34.5±14.7	31±13.6	35±12.2
Group D	40±13.78	59.5±23.82^a	41.5±16.89^a	53.5±17.76^a

Notes: ^aStatistically significant results for MG loss.

Abbreviations: MG, meibomian gland; RUL, right upper lid; RLL, right lower lid; LUL, left upper lid; LLL, left lower lid.