Reproducibility of retinal circulation measurements obtained using laser speckle flowgraphy-NAVI in patients with glaucoma

Figures & data

Table 1 Demographic data for patients with or without glaucoma

	GHT+ (n = 38)	GHT− (n = 27)	P value
Number of subjects	38	27
Age (years)	48.9 ± 12.8	50.2 ± 8.7	0.60
Gender (males:females)	20/18	14/13	0.95
Visual acuity (logMAR)	−0.02 ± 0.15	−0.06 ± 0.06	0.41
Spherical equivalent (D)	−4.2 ± 2.7	−3.8 ± 2.8	0.59
MD (dB)	−7.2 ± 7.2	−0.4 ± 1.4	<0.0001
Intraocular pressure (mmHg)	17.1 ± 3.4	17.5 ± 5.2	0.69
Systolic blood pressure (mmHg)	123.1 ± 14.7	125.1 ± 13.8	0.28
Diastolic blood pressure (mmHg)	74.5 ± 14.2	74.0 ± 12.3	0.88
Heart rate (per minutes)	73.6 ± 11.0	70.2 ± 9.9	0.23

Figure 1 Representative photographs of a glaucoma patient in this study. (A) Photograph of the fundus. (B) A representative color map produced using LSFG (1, retinal artery; 2, retinal vein; 3, optic disk; 4, choroid). (C) Representative binary format images for segmentation between the vessel (white area) and tissue (black area), obtained using image viewer software with an automated definitive threshold. (D) Orientation of the quadrant assessment for the MBR in the optic disk.

Table 2 Intrasession reproducibility for GHT− and GHT+ subjects

MBR	GHT−		GHT+
	COV	ICC	COV	ICC
Artery	8.7 ± 4.5	0.90	12.4 ± 12.4	0.82
Vein	6.2 ± 3.5	0.90	10.1 ± 6.3	0.81
Choroid	4.1 ± 3.2	0.98	5.2 ± 3.7	0.95
Disc-AM	2.9 ± 2.1	0.95	3.8 ± 2.0	0.97
Disc-VM	1.9 ± 1.1	0.98	2.6 ± 1.8	0.96
Disc-TM	2.1 ± 1.1	0.97	2.3 ± 1.5	0.98
I-AM	4.2 ± 2.9	0.98	5.8 ± 4.1	0.93
I-VM	2.8 ± 2.0	0.98	3.5 ± 1.9	0.94
I-TM	2.9 ± 2.1	0.97	3.5 ± 2.7	0.96
T-AM	3.4 ± 1.9	0.99	4.2 ± 2.3	0.98
T-VM	2.3 ± 1.2	0.93	4.2 ± 3.8	0.96
T-TM	3.0 ± 2.1	0.98	3.7 ± 2.3	0.97
S-AM	3.6 ± 2.1	0.98	6.4 ± 4.4	0.94
S-VM	2.3 ± 1.3	0.98	3.5 ± 2.8	0.93
S-TM	2.9 ± 2.1	0.96	4.6 ± 2.9	0.95
N-AM	4.7 ± 4.4	0.92	5.1 ± 4.2	0.94
N-VM	2.3 ± 1.7	0.98	2.6 ± 1.9	0.97
N-TM	3.6 ± 2.5	0.92	3.8 ± 3.2	0.95

Abbrevations: AM, mean of all MBRs; COV, coefficient of variation; GHT, Glaucoma Hemifield Test; I, inferior quadrant area of optic disk; ICC, intraclass correlation coefficient; MBR, mean blur rate; N, nasal quadrant area of optic disk; S, superior quadrant area of optic disk; T, temporal quadrant area of optic disk; TM, tissue mean; VM, vessel mean.