Expression of interleukin-6 in ocular surface squamous neoplasia

Figures & data

Table 1 Immunohistochemical staining for interleukin-6 in cytoplasm and nucleus in non-neoplastic and ocular surface squamous neoplasia (OSSN) tissues. Note that the most intense staining (+3) in either cytoplasm or nucleus was observed in OSSN cases

Group Staining intensity		OSSN N (%)	Control N (%)	P-Value
Cytoplasm	0	0	3 (15)	<0.0001
	+1	3 (15)	14 (70)
	+2	7 (35)	3 (15)
	+3	10 (50)	0
Nucleus	0	1 (5)	4 (20)	<0.003
	+1	4 (20)	9 (45)
	+2	6 (30)	6 (30)
	+3	9 (45)	1 (5)
Total		20 (100)	20 (100)

Note: N, number of samples.

Table 2 Staining distribution in the nucleus and cytoplasm in different grades of dysplasia

Staining Histology	CIN I	CIN II	CIN III	SCC	P-value
Nucleus					0.766
0	0	0	1	0
+1	0	1	1	2
+2	2	1	0	3
+3	1	3	2	3
Cytoplasm					0.079
0	0	0	0	0
+1	1	1	1	0
+2	2	2	2	1
+3	0	2	1	7

Figure 1 (A–F) H&E versus Immunohistochemistry stainings for interleukin-6 in samples of OSSN and conjunctival tissue. (A, B): Non-neoplastic conjunctiva; (C, D): Conjunctival intraepithelial neoplasia Grade III: note the positive cytoplasmic staining; (E, F): Squamous cell carcinoma shows strong positive staining. Scale bar is 100 µ.

Abbreviations: H&E, hematoxylin and eosin; OSSN, ocular surface squamous neoplasia.

Figure 2 (A–D) Intense nuclear staining in OSSN(Right) and control group (Left). Moderate intensity nuclear staining is specially observed in the basal epithelium of control group. Significance of nuclear staining is not clear. (A, C): H&E staining, (B, D): Immunohistochemistry staining for interleukin-6. Scale bar is 100 µm.

Abbreviations: OSSN, ocular surface squamous neoplasia; H&E, hematoxylin and eosin.