Comparison of 3D Digitally Assisted Visualization System with Current Standard Visualization for the Removal of Vitreous in a Preclinical Model

Figures & data

Table 1 Equipment Settings

	Arm 1 NGENUITY 3D DAVS	Arm 2 Standard Microscope
Vitrectomy platform	CONSTELLATION Vision System	CONSTELLATION Vision System
Visualization system	LuxOR LX3 with NGENUITY 3D DAVS	LuxOR LX3 with standard binocular
Vitrectomy optics	Oculus BIOM 200 mm	Oculus BIOM 200 mm
Optimal magnification	× 5	× 5

Abbreviation: DAVS, digitally assisted visualization system.

Table 2 NGENUITY 3D DAVS Settings Applied During Surgery

Parameters	NGENUITY 3D DAVS Settings
Camera	Standard
Light profile	Xenon
Gain	1
Channel	Off
RGB	100, 100, 100
Brightness	49.00
Contrast	52.50
Gamma	1.00
Hue	32
Saturation	87

Abbreviations: DAVS, digitally assisted visualization system; RGB, red green blue.

Figure 1 Residual vitreous assessment. Posterior segment of the eye was removed (A), the eye was bisected (B), and residual vitreous was stripped (C and D).

Figure 2 Comparison of NGENUITY 3D DAVS and a standard visualization system for the removal of vitreous. Mean residual vitreous (A) and mean percentage of vitreous removed (B) are shown (n = 15). *P < 0.0001.

Abbreviation: 3D DAVS, 3D digitally assisted visualization system.

Table 3 Comparison of OD and OS

	Mean ± SD Vitreous Weight, g	Mean ± SD Axial Length, mm	Difference Between OD and OS
			Mean ± SD Difference in Vitreous Weight, g	Percentage Difference in Vitreous Weight, %
OD	2.47 ± 0.15	17.48 ± 0.19	0.046 ± 0.035	1.87
OS	2.49 ± 0.15	17.54 ± 0.20

Abbreviations: OD, right eye; OS, left eye.

Table 4 Vitreous Weight and Axial Length for Contralateral Eyes in Comparison Analysis

Animal	Vitreous Weight, g			Axial Length, mm
	OD	OS	Difference Between OD and OS, g	OD	OS	Difference Between OD and OS, mm
1	2.1517	2.2378	0.0861	17.46	17.18	0.28
2	2.4288	2.3884	0.0404	17.19	17.28	0.09
3	2.5095	2.5925	0.0830	17.82	17.71	0.11
4	2.7157	2.7145	0.0012	17.69	17.72	0.03
5	2.5450	2.5368	0.0082	17.42	17.53	0.11
6	2.3515	2.3408	0.0107	17.60	17.80	0.20
7	2.4919	2.5024	0.0105	17.55	17.60	0.05
8	2.3787	2.4473	0.0686	17.61	17.77	0.16
9	2.4122	2.5300	0.1178	17.52	17.55	0.03
10	2.4118	2.3767	0.0351	17.27	17.36	0.09
11	2.3791	2.3124	0.0667	17.21	17.34	0.13
12	2.6853	2.7471	0.0618	17.66	17.66	0
13	2.5239	2.4871	0.0368	17.45	17.68	0.23
14	2.6228	2.6008	0.0220	17.32	17.34	0.02
Mean (SD)	2.47 (0.15)	2.49 (0.15)	0.0464 (0.0352)	17.48 (0.18)	17.54 (0.20)	0.11 (0.08)
P value (OD vs OS)			0.35			0.15

Note: P values were calculated using paired t-test.

Abbreviations: OD, right eye; OS, left eye.

Figure 3 Vitreous visualization using NGENUITY 3D digitally assisted visualization system alternated between right eye and left eye to reduce surgical bias; vitreous was highlighted in a blue color.