Acute hemorrhagic conjunctivitis: anti-coxsackievirus A24 variant secretory immunoglobulin A in acute and convalescent tear

Figures & data

Figure 1 Clinical presentation of bilateral ocular CA24v infection.

Notes: (A) First day morning; (B) first day afternoon; (C) second day am; (D) second day pm; (E) third day am; (F) fourth day pm; (G) sixth day pm. The bilateral lid edema (*), vascular dilation, and conjunctival erythema were noted on the second day, and the inferior-nasal chemosis (arrow) was present on the third day post-AHC. The clinical signs were less and resolved more quickly in the right (second infected) than the left (first affected) eye.
Abbreviations: CA24v, coxsackievirus A24; AHC, acute hemorrhagic conjunctivitis.

Figure 2 Anti-CA24v neutralizing titers in acute tears post-AHC onset.

Abbreviations: CA24v, coxsackievirus A24 variant; AHC, acute hemorrhagic conjunctivitis.

Figure 3 Anti-CA24v serum antibody response.

Notes: Serum neutralization titer against CA24v isolates; Louisiana/LTV/2010, Texas/MO7/1977, and prototype Singapore/SEC24/1970.
Abbreviation: CA24v, coxsackievirus A24 variant.

Figure 4 Immunoreactivity of tear and serum IgG (green) and IgA (red) from the AHC patient with CA24v-infected cells.

Notes: Arrows show similar differential staining patterns for tear IgG and IgA in CA24v infected HRPE and AGMK cells. (A) Detection of immunoreactive human IgG and/or IgA in CA24v-infected HRPE cells treated with pooled acute tears (collected 1–4 days po). (B) Detection of immunoreactive human IgG and/or IgA in CA24v-infected AGMK cells treated with pooled acute tears. (C) Detection of immunoreactive human IgG and IgA in CA24v-infected HRPE cells treated with tears collected 4 years post-AHC. (D) Detection of immunoreactive human IgG and IgA in CA24v-infected AGMK cells treated with tears collected 5 years post-AHC. (E) Detection of antibody to secretory component (polymeric IgA) in CA24v-infected HRPE cells treated with pooled acute tears. (F) Detection of antibody to secretory component (polymeric IgA) in CA24v-infected HRPE cells treated with tears collected 5 years post-AHC. (G) Detection of CA24v immunoreactive human IgG and IgA in acute serum (collected 11 days po)-treated virus-infected HRPE cells. (H) Detection of CA24v immunoreactive human IgG and IgA in virus-infected HRPE cells treated with the patient’s convalescent serum (collected 5 years post-AHC). Bar =50 µm.
Abbreviations: IgG, immunoglobulin G; IgA, immunoglobulin A; AHC, acute hemorrhagic conjunctivitis; CA24v, coxsackievirus A24 variant; HRPE, human retinal pigmented epithelial; po, post-onset; AGMK, African green monkey kidney.