Figures & data
Figure 1 Histological characteristics of normal and osteosarcoma tissues.
Notes: (A) Histological section of normal bone tissue of a mouse showing uniform arrangement of cells. (B) Primary osteosarcoma tissue with abnormal mass of cells. (C) Metastatic osteosarcoma tissue showing irregular cells that are enlarged in size when compared to normal cells. (D) Primary osteosarcoma tissue treated with SAM showing reversion in cellular pattern. (E) Metastatic osteosarcoma tissue treated with SAM showing no changes in cellular pattern. Scale bar: 100 µm.
Abbreviation: SAM, S-adenosylmethionine.
Abbreviation: SAM, S-adenosylmethionine.
Figure 2 Analysis of Sox2 expression in normal and osteosarcoma tissues.
Notes: (A) Sox2 expression in normal bone tissue of a mouse. (B) Sox2 expression in primary osteosarcoma tissue. (C) Sox2 expression in metastatic osteosarcoma tissue. (D) Sox2 expression in SAM-treated primary osteosarcoma tissue. (E) Sox2 expression in SAM-treated metastatic osteosarcoma tissue. Scale bar: 100 µm.
Abbreviation: SAM, S-adenosylmethionine.
Abbreviation: SAM, S-adenosylmethionine.
Figure 3 Analysis of TCTP expression in normal and osteosarcoma tissues.
Notes: (A) TCTP expression in normal bone tissue of a mouse. (B) TCTP expression in primary osteosarcoma tissue. (C) TCTP expression in metastatic osteosarcoma tissue. (D) Increased TCTP expression in SAM-treated primary osteosarcoma tissue. (E) TCTP expression in SAM-treated metastatic osteosarcoma tissue. Scale bar: 100 µm.
Abbreviation: SAM, S-adenosylmethionine.
Abbreviation: SAM, S-adenosylmethionine.
Figure 4 Western blotting analysis of Sox2 and TCTP expression.
Notes: The protein samples were prepared from SAM-treated osteosarcoma tissue along with various controls and subjected to Western blotting analysis and then stained with anti-Sox2 antibody and anti-TCTP antibody. Lane 1 shows Sox2 and TCTP expression in normal bone tissue samples of mice. Lane 2 represents Sox2 and TCTP expression in primary osteosarcoma tissue samples. Lane 3 represents Sox2 and TCTP expression in metastatic osteosarcoma tissue samples. Lane 4 shows Sox2 and TCTP expression in SAM-treated primary osteosarcoma tissue samples. Lane 5 represents Sox2 and TCTP expression in SAM-treated metastatic osteosarcoma tissue samples. Tubulin was used as a loading control.
Abbreviation: SAM, S-adenosylmethionine.
Abbreviation: SAM, S-adenosylmethionine.
