Figures & data
Figure 2 MTT assay growth curve of PC3 cells treated with biseugenol B at 24, 48, and 72 hours.
![Figure 2 MTT assay growth curve of PC3 cells treated with biseugenol B at 24, 48, and 72 hours.](/cms/asset/90de67ed-ec8d-4d3f-82b4-6c67ab0b6f2c/dott_a_12195364_f0002_c.jpg)
Table 1 IC50 concentration of biseugenol B
Figure 3 AO-PI double-staining cell morphological analysis in untreated and treated PC3 cells with biseugenol B.
Abbreviations: AO, acridine orange; VI, viable cells; EA, early apoptosis; LA, late apoptosis; SN, secondary necrosis; PI, propidium iodide.
![Figure 3 AO-PI double-staining cell morphological analysis in untreated and treated PC3 cells with biseugenol B.](/cms/asset/2508adb7-1998-4752-87a1-d90bdb421da9/dott_a_12195364_f0003_c.jpg)
Figure 4 The percentages of VI, EA, LA and SN cells after biseugenol B treatment.
Abbreviations: VI, viable cells; EA, early apoptosis; LA, late apoptosis; SN, secondary necrosis; SD, standard deviation.
![Figure 4 The percentages of VI, EA, LA and SN cells after biseugenol B treatment.](/cms/asset/add39aab-5897-4aa6-bfaa-a47d07944012/dott_a_12195364_f0004_c.jpg)
Figure 5 The effect of biseugenol B on EA and LA of PC3 cells.
Abbreviations: EA, early apoptosis; LA, late apoptosis; FITC, fluorescein isothiocyanate; AV, annexin V; PI, propidium iodide; VI, viable cells; SD, standard deviation.
![Figure 5 The effect of biseugenol B on EA and LA of PC3 cells.](/cms/asset/e009ddca-31ca-433f-9976-3ef8d6ea1e65/dott_a_12195364_f0005_c.jpg)
Figure 6 Cell cycle histogram from analyses of PC3 cells treated with 0 µg/mL (A), 2 µg/mL (B), 4 µg/mL (C) and 8 µg/mL (D) of biseugenol B for 24 hours. (E) Summary of cell cycle progression for control and biseugenol B-treated PC3 cells.
Abbreviation: SD, standard deviation.
![Figure 6 Cell cycle histogram from analyses of PC3 cells treated with 0 µg/mL (A), 2 µg/mL (B), 4 µg/mL (C) and 8 µg/mL (D) of biseugenol B for 24 hours. (E) Summary of cell cycle progression for control and biseugenol B-treated PC3 cells.](/cms/asset/26b38858-0549-4771-b863-9a611fa5beac/dott_a_12195364_f0006_c.jpg)
Table 2 Effect of biseugenol B on cell cycle phases
Figure 7 The effect of biseugenol B on nuclear size, MMP, cell membrane permeability, and cytochrome c release in PC3.
Abbreviations: MMP, mitochondrial membrane potential; SD, standard deviation.
![Figure 7 The effect of biseugenol B on nuclear size, MMP, cell membrane permeability, and cytochrome c release in PC3.](/cms/asset/eecb5c9e-cae1-4cbb-8fa9-e722e0971b02/dott_a_12195364_f0007_c.jpg)
Figure 8 Relative bioluminescence expression of caspase-3/7, caspase-8, and caspase-9 in PC3 and RWPE-1 cells treated with biseugenol B.
Abbreviation: SD, standard deviation.
![Figure 8 Relative bioluminescence expression of caspase-3/7, caspase-8, and caspase-9 in PC3 and RWPE-1 cells treated with biseugenol B.](/cms/asset/92baa2eb-b084-473d-b1f0-89a669c7f5c5/dott_a_12195364_f0008_c.jpg)
Figure 9 DCF-fluorescence intensity after exposure of biseugenol B for 24 hours in PC3 and RWPE1.
Abbreviations: ROS, reactive oxygen species; DCF, dichlorodihydrofluorescein; SD, standard deviation.
![Figure 9 DCF-fluorescence intensity after exposure of biseugenol B for 24 hours in PC3 and RWPE1.](/cms/asset/dc60014b-623a-4fed-9776-108ea2091e03/dott_a_12195364_f0009_c.jpg)
Figure 10 Effects of biseugenol B on the Bax, Bcl-2 and Hsp70 mRNA expression level in PC3 cells.
Abbreviations: Bax, Bcl-2-associated X; Bcl-2, Bcl-cell lymphoma-2; Hsp70, heat-shock protein 70; PCR, polymerase chain reaction; SD, standard deviation.
![Figure 10 Effects of biseugenol B on the Bax, Bcl-2 and Hsp70 mRNA expression level in PC3 cells.](/cms/asset/bd05a872-1063-432f-a59a-30f45f68bd17/dott_a_12195364_f0010_b.jpg)
Figure 11 Western blot analysis of biseugenol B in the selected apoptotic signaling markers.
Abbreviations: Bax, Bcl-2-associated X; Bcl-2, Bcl-cell lymphoma-2; Hsp70, heat-shock protein 70; SD, standard deviation.
![Figure 11 Western blot analysis of biseugenol B in the selected apoptotic signaling markers.](/cms/asset/ff1d0695-4992-40d6-aa1b-390ec9513305/dott_a_12195364_f0011_c.jpg)
Figure 12 Inhibition of TNF-α-induced NF-κB nuclear translocation by biseugenol B.
Abbreviations: TNF-α, tumor necrosis factor-alpha; NF-κB, nuclear factor kappa-B.
![Figure 12 Inhibition of TNF-α-induced NF-κB nuclear translocation by biseugenol B.](/cms/asset/c3efbd5a-ceed-4f4c-9449-dbc2371f720c/dott_a_12195364_f0012_c.jpg)
Figure 13 Immunoblot analysis of nuclear NF-κB.
Abbreviations: NF-κB, nuclear factor kappa-B; SD, standard deviation.
![Figure 13 Immunoblot analysis of nuclear NF-κB.](/cms/asset/23c5ca48-a01b-40bb-9c64-9835bc005977/dott_a_12195364_f0013_c.jpg)