Decreased miR-503 expression in gastric cancer is inversely correlated with serum carcinoembryonic antigen and acts as a potential prognostic and diagnostic biomarker

Figures & data

Table 1 Correlations between serum miR-503 expression levels and clinical parameters

Variables	GC patients (n=68)	Healthy controls (n=30)	P-value
Age (years)			0.237
<60	25	16
≥60	43	14
Sex			0.205
Male	43	20
Female	25	10
Tumor size (cm)			0.132
<5	40
≥5	28
Histological grade			0.394
Well and moderate	19
Poor and others	49
Depth of tumor			0.152
T1, T2	17
T3, T4	51
Lymphatic metastasis			0.029*
Absent	27
Present	41
Distant metastasis			0.036
Absent	52
Present	16
Tumor stagea			0.031*
I and II	24
III and IV	44

Notes:

a According to the TNM criteria.

* P<0.05.

Abbreviation: GC, gastric cancer.

Figure 1 MiR-503 downregulation in GC tissues.

Notes: (A) The expression level of miR-503 in GC and adjacent normal mucosal tissues (n=68) was determined by RT-qPCR analysis. (B) MiR-503 expression levels in metastatic (n=54) and nonmetastatic (n=14) GC.
Abbreviations: GC, gastric cancer; RT-qPCR, real-time reverse transcription quantitative polymerase chain reaction.

Figure 2 Diagnostic and prognostic significance of miR-503 in tissues of GC.

Notes: (A) ROC curve analysis showing performance of miR-503 expression to discriminate between malignant and nonmalignant tissue samples. (B) Kaplan–Meier analysis and log-rank test for overall survival of GC patients based on miR-503 expression.
Abbreviations: GC, gastric cancer; ROC, receiver operating characteristic; AUC, area under the curve; CI, confidence interval.

Figure 3 MiR-503 expression and diagnostic significance in serum of GC patients and healthy controls.

Notes: (A) The expression level of miR-503 in serum of GC patients (n=32) or healthy participants (n=32) was determined by RT-qPCR analysis. (B) The expression level of CEA in serum of GC patients (n=32) or healthy participants (n=32) was determined by RT-qPCR analysis. (C). Correlation of the expression of miR-503 and CEA in GC patients. (D) ROC analysis curve of CEA and miR-503 expression in the serum of the GC patients and healthy controls.
Abbreviations: GC, gastric cancer; RT-qPCR, real-time reverse transcription quantitative polymerase chain reaction; CEA, carcinoembryonic antigen; ROC, receiver operating characteristic; AUC, area under the curve; CI, confidence interval.