Figures & data
Figure 1 The expression of miR-543 was downregulated in breast cancer tissues.
Notes: (A) The level of miR-543 was decreased in cancer tissues when compared with adjacent tissues according to the results detected in 20 paired clinical samples. (B) The level of miR-543 was lower in cancer site on the basis of in situ hybridization assay. (C) The typical pictures for in situ hybridization assay, expression of miR-543 was lower in cancer site section. (D) The expression of miR-543 in 4 breast cancer cell lines. n=3, **P<0.01 vs cancer tissues or MDA-MB-231.
Figure 2 High expression of miR-543 inhibits cell proliferation, induces cell apoptosis and blocks cell cycle.
Notes: (A) miR-543 overexpression and knocking down cell models were established successfully. (B) Cell proliferation was inhibited when miR-543 was overexpressed. (C) The capability of clone formation was restrained in miR-543 mimics group. (D) Cell apoptosis rate was increased in miR-543 mimics group. (E) Cell cycle was suppressed when miR-543 was overexpressed. (F) Protein expression level of CyclinD1 and Bcl-2 was downregulated and BAX was increased in miR-543 mimics group. n=5, *P<0.05, **P<0.01 vs control or NC group.
Abbreviation: NC, nitrocellulose.
Abbreviation: NC, nitrocellulose.
Figure 3 ERK2 is a gene target of miR-543.
Notes: (A) The mRNA expression level of ERK2 in 4 breast cancer cell lines. (B) The relationship between ERK2 and miR-543 was assessed by Luciferase reporter assay. (C) The expression of MDA-MB-231 and MCF-7 in different groups n=5, *P<0.05, **P<0.01 vs MDA-MB-231 or mutant group.
Abbreviations: ERK, extracellular signal-regulated kinase; UTR, untranslated region.
Abbreviations: ERK, extracellular signal-regulated kinase; UTR, untranslated region.
Figure 4 The effect of miR-543 on breast cancer was achieved through the regulation of MAPK/ERK signal pathway.
Notes: (A) The activity of MAPK/ERK was detected by Western blot. (B) Chemotherapy resistance was suppressed in miR-543 mimics group. (C) Inhibited clone formation in miR-543 mimics group. (D) Cell apoptosis tested by flow cytometry. (E) Cell cycle results tested by flow cytometry. (E) The protein level of CyclinD1, Bcl-2 and BAX was measured by Western blot. n=5, *P<0.05, **P<0.01 vs mimics NC group or as marked in the figure.
Abbreviations: ERK, extracellular signal-regulated kinase; MAPK, mitogen-activated protein kinase; NC, nitrocellulose.
Abbreviations: ERK, extracellular signal-regulated kinase; MAPK, mitogen-activated protein kinase; NC, nitrocellulose.
