No genetic relationship between TLR2 rs4696480, rs3804100, and rs3804099 gene polymorphisms and female breast cancer in Saudi populations

Figures & data

Table 1 Clinical characteristics of Saudi BC cases and healthy controls used for genotyping

Variable	Character	Patients, n	Controls, n
Total people	na	126	146
Age (years), median age (48±8.2)	≤48 years	44	57
	>48 years	82	55
ER	ER+	75	na
	ER−	49	na
PR	PR+	71	na
	PR−	55	na
HER status	HER+	49	na
	HER−	78	na

Abbreviations: BC, breast cancer; ER, estrogen receptor; PR, progesterone receptor; HER, human epidermal growth factor receptor; na, not applicable.

Table 2 Distribution of genotypes and allele frequencies of the TLR2 gene loci among Saudi BC patients and healthy controls

Genotype	Cases, n	HWE P-value	Controls, n	HWE P-value
rs3804100
CC	1	0.728395	4	0.051917
CT	24		27
TT	99		115
rs4696480
AA	29	0.306821	25	0.048883
AT	51		63
TT	46		50
rs3804099
CC	32	0.480478	42	0.424288
CT	58		71
TT	35		33

Abbreviations: TLR2, Toll-like receptor 2; BC, breast cancer; HWE, Hardy–Weinberg equilibrium.

Table 3 Genotype frequencies of TLR2 gene polymorphism in Saudi BC patients and healthy controls

Gene	SNP ID	Genotype	Breast, n (%)	Controls, n (%)	OR	95% CI	χ^2-value	P-value
TLR2	rs3804100		n=124	n=146
		CC	1 (0.01)	4 (0.03)	Ref
		CT	24 (0.19)	27 (0.18)	4.800	0.496–46.472	2.14	0.14312
		TT	99 (0.80)	115 (0.79)	4.500	0.494–41.022	2.12	0.14563
		CT + TT	123 (0.99)	142 (0.97)	4.556	0.502–41.382	2.17	0.14078
		C	26 (0.10)	35 (0.12)	Ref
		T	222 (0.90)	257 (0.88)	1.220	0.692–2.151	0.47	0.49199
	rs4696480		n=126	n=138	OR	95% CI	χ^2-value	P-value
		AA	29 (0.23)	25 (0.18)	Ref
		AT	51 (0.40)	63 (0.46)	0.769	0.387–1.528	0.56	0.45350
		TT	46 (0.37)	50 (0.36)	0.812	0.403–1.639	0.34	0.56167
		AT + TT	97 (0.77)	113 (0.82)	0.789	0.420–1.483	0.54	0.46168
		A	109 (0.43)	113 (0.41)	Ref
		T	143 (0.57)	163 (0.59)	0.908	0.630–1.310	0.26	0.60681
	rs3804099		n=125	n=146	OR	95% CI	χ^2-value	P-value
		CC	32 (0.26)	42 (0.29)	Ref
		CT	58 (0.46)	71 (0.48)	1.185	0.643–2.183	0.30	0.58569
		TT	35 (0.28)	33 (0.22)	1.430	0.710–2.881	1.01	0.31593
		CT + TT	93 (0.74)	104 (0.71)	1.267	0.717–2.238	0.67	0.41469
		C	122 (0.49)	155 (0.53)	Ref
		T	128 (0.51)	137 (0.47)	1.211	0.844–1.737	1.08	0.29951

Abbreviations: TLR2, Toll-like receptor 2; BC, breast cancer; SNP, single-nucleotide polymorphism; OR, odds ratio; CI, confidence interval; Ref, reference.

Table 4 Comparison of genotype frequencies of SNPs in the TLR2 gene between Saudi BC cases (patient age ≤48 years) and healthy controls

Gene	SNP ID	Genotype	Breast, n (%)	Controls, n (%)	OR	95% CI	χ^2-value	P-value
TLR2	rs3804100		n=43	n=57
		CC	1 (0.02)	3 (0.05)	Ref
		CT	11 (0.26)	11 (0.20)	3.000	0.27–33.48	0.85	0.35622
		TT	31 (0.72)	43 (0.75)	2.163	0.21–21.78	0.45	0.50350
		CT + TT	42 (0.98)	54 (0.95)	2.333	0.234–23.246	0.55	0.45799
		C	13 (0.15)	17 (0.15)	Ref
		T	73 (0.85)	97 (0.85)	0.984	0.450–2.154	0.00	0.96809
	rs4696480		n=44	n=57	OR	95% CI	χ^2-value	P-value
		AA	10 (0.23)	12 (0.21)	Ref
		AT	18 (0.41)	24 (0.42)	0.900	0.319–2.542	0.04	0.84231
		TT	16 (0.36)	21 (0.37)	0.914	0.316–2.644	0.03	0.86860
		AT + TT	34 (0.77)	45 (0.79)	0.907	0.351–2.345	0.04	0.83978
		A	38 (0.43)	48 (0.42)	Ref
		T	50 (0.57)	66 (0.58)	0.957	0.545–1.679	0.02	0.87805
	rs3804099		n=44	n=57	OR	95% CI	χ^2-value	P-value
		CC	10 (0.23)	16 (0.28)	Ref
		CT	25 (0.57)	28 (0.49)	1.429	0.549–3.718	0.54	0.46407
		TT	9 (0.20)	13 (0.23)	1.108	0.347–3.535	0.03	0.86283
		CT + TT	34 (0.77)	41 (0.72)	1.327	0.533–3.301	0.37	0.54255
		C	45 (0.51)	60 (0.53)	Ref
		T	43 (0.49)	54 (0.47)	1.062	0.609–1.853	0.04	0.83296

Abbreviations: SNP, single-nucleotide polymorphism; TLR2, Toll-like receptor 2; BC, breast cancer; OR, odds ratio; CI, confidence interval; Ref, reference.

Table 5 Comparison of genotype frequencies of SNPs in the TLR2 gene between Saudi BC cases (patient age >48 years) and healthy controls

Gene	SNP ID	Genotype	Breast, n (%)	Controls, n (%)	OR	95% CI	χ^2-value	P-value
TLR2	rs3804100		n=81	n=55
		CC	0 (0.00)	1 (0.02)	Ref
		CT	13 (0.16)	9 (0.16)	4.263	0.156–116.34	1.36	0.24370
		TT	68 (0.84)	45 (0.82)	4.516	0.18–113.32	1.49	0.22201
		CT + TT	81 (1)	54 (0.98)	4.486	0.179–112.162	1.48	0.22321
		C	13 (0.08)	11 (0.10)	Ref
		T	149 (0.92)	99 (0.90)	1.274	0.549–2.956	0.32	0.57296
	rs4696480		n=82	n=53	OR	95% CI	χ^2-value	P-value
		AA	19 (0.23)	9 (0.17)	Ref
		AT	33 (0.40)	24 (0.45)	0.651	0.251–1.687	0.78	0.37573
		TT	30 (0.37)	20 (0.38)	0.711	0.268–1.882	0.47	0.49096
		AT + TT	63 (0.77)	44 (0.83)	0.678	0.281–1.638	0.75	0.38640
		A	71 (0.43)	42 (0.40)	Ref
		T	93 (0.57)	64 (0.60)	0.860	0.523–1.413	0.36	0.55054
	rs3804099		n=81	n=55	OR	95% CI	χ^2-value	P-value
		CC	22 (0.27)	18 (0.33)	Ref
		CT	33 (0.41)	24 (0.44)	1.125	0.498–2.542	0.08	0.77699
		TT	26 (0.32)	13 (0.23)	1.636	0.658–4.072	1.13	0.28835
		CT + TT	59 (0.73)	37 (0.67)	1.305	0.619–2.752	0.49	0.48440
		C	77 (0.48)	60 (0.55)	Ref
		T	85 (0.52)	50 (0.45)	1.325	0.815–2.153	1.29	0.25614

Abbreviations: SNP, single-nucleotide polymorphism; TLR2, Toll-like receptor 2; BC, breast cancer; OR, odds ratio; CI, confidence interval; Ref, reference.

Table 6 Comparison of genotype frequencies of SNPs in the TLR2 gene between ER +ve Saudi BC cases and healthy controls

Gene	SNP ID	Genotype	Breast, n (%)	Controls, n (%)	OR	95% CI	χ^2-value	P-value
TLR2	rs3804100		n=75	n=146
		CC	1 (0.01)	4 (0.03)	Ref
		CT	15 (0.2)	27 (0.18)	3.000	0.303–29.663	0.95	0.32911
		TT	59 (0.79)	115 (0.79)	2.682	0.292–24.593	0.82	0.36500
		CT + TT	74 (0.99)	142 (0.97)	2.741	0.300–25.013	0.86	0.35244
		C	17 (0.11)	35 (0.12)	Ref
		T	133 (0.89)	257 (0.88)	1.118	0.588–2.123	0.12	0.73393
	rs4696480		n=75	n=138	OR	95% CI	χ^2-value	P-value
		AA	16 (0.21)	25 (0.18)	Ref
		AT	34 (0.45)	63 (0.46)	0.930	0.424–2.038	0.03	0.85552
		TT	25 (0.34)	50 (0.36)	0.800	0.353–1.815	0.28	0.59361
		AT + TT	59 (0.79)	113 (0.82)	0.870	0.420–1.804	0.14	0.70813
		A	66 (0.44)	113 (0.41)	Ref
		T	84 (0.56)	163 (0.59)	0.881	0.579–1.341	0.35	0.55444
	rs3804099		n=75	n=146	OR	95% CI	χ^2-value	P-value
		CC	21 (0.28)	42 (0.29)	Ref
		CT	36 (0.48)	71 (0.49)	1.121	0.562–2.235	0.11	0.74589
		TT	18 (0.24)	33 (0.22)	1.121	0.498–2.521	0.08	0.78258
		CT + TT	54 (0.72)	104 (0.71)	1.121	0.588–2.137	0.12	0.72880
		C	78 (0.52)	155 (0.53)	Ref
		T	72 (0.48)	137 (0.47)	1.065	0.704–1.612	0.09	0.76539

Abbreviations: SNP, single-nucleotide polymorphism; TLR2, Toll-like receptor 2; ER, estrogen receptor; BC, breast cancer; OR, odds ratio; CI, confidence interval; Ref, reference.

Table 7 Comparison of genotype frequencies of SNPs in the TLR2 gene between ER −ve Saudi BC cases and healthy controls

Gene	SNP ID	Genotype	Breast, n (%)	Controls, n (%)	OR	95% CI	χ^2-value	P-value
TLR2	rs3804100		n=47	n=146
		CC	0 (0.00)	4 (0.03)	Ref
		CT	8 (0.17)	27 (0.18)	3.732	0.180–77.172	1.52	0.21704
		TT	39 (0.83)	115 (0.79)	4.017	0.211–76.419	1.75	0.18600
		CT + TT	47 (1)	142 (0.97)	0.18600	0.208–74.648	1.72	0.18945
		C	8 (0.09)	35 (0.12)	Ref
		T	86 (0.91)	257 (0.88)	1.536	0.673–3.506	1.05	0.30558
	rs4696480		n=49	n=138	OR	95% CI	χ^2-value	P-value
		AA	12 (0.24)	25 (0.18)	Ref
		AT	16 (0.33)	63 (0.46)	0.583	0.235–1.445	1.37	0.24194
		TT	21 (0.43)	50 (0.36)	0.896	0.371–2.167	0.06	0.80804
		AT + TT	37 (0.76)	113 (0.82)	0.728	0.325–1.629	0.60	0.43828
		A	40 (0.41)	113 (0.41)	Ref
		T	58 (0.59)	163 (0.59)	1.004	0.619–1.629	0.00	0.98760
	rs3804099		n=48	n=146	OR	95% CI	χ^2-value	P-value
		CC	9 (0.19)	42 (0.29)	Ref
		CT	22 (0.46)	71 (0.49)	1.598	0.658–3.884	1.08	0.29841
		TT	17 (0.35)	33 (0.22)	2.470	0.950–6.424	3.53	0.06033
		CT + TT	39 (0.81)	104 (0.71)	1.889	0.824–4.328	2.30	0.12909
		C	40 (0.42)	155 (0.53)	Ref
		T	56 (0.58)	137 (0.47)	1.615	0.996–2.619	3.81	0.05096

Abbreviations: SNP, single-nucleotide polymorphism; TLR2, Toll-like receptor 2; ER, estrogen receptor; BC, breast cancer; OR, odds ratio; CI, confidence interval; Ref, reference.

Table S1 Characteristics of selected polymorphisms in the TLR2

SNP ID	Chromosome position	Nucleotide change	Region
rs3804100
Ser450Ser	4/153704257	+1350C>T	Exon
rs4696480
	4/153685974	−16934T>A	Promoter
rs3804099
Asn199Asn	4/153703504	+597T>C	Exon

Abbreviations: TLR2, Toll-like receptor 2; SNP, single-nucleotide polymorphism.