Prognostic significance of microRNA-100 in solid tumors: an updated meta-analysis

Figures & data

Figure 1 Flow diagram of filtering studies.

Abbreviations: NOS, Newcastle–Ottawa scale; OS, overall survival.

Table 1 Newcastle–Ottawa scale

	Selection	Comparability	Outcome	Total
Feber et al^Citation12	4	0e,f	2g	6
Huang et al^Citation13	4	0e,f	2g	6
Torres et al^Citation16	4	0e,f	2g	6
Peng et al^Citation17	4	1e	2g	7
Wang et al^Citation19	4	1e	2g	7
Liu et al^Citation14	4	1e	2g	7
Wang et al^Citation21	4	1e	2g	7
Sun et al^Citation22	4	2	1g,h	7
Chen et al^Citation24	4	1e	2g	7
Chen et al^Citation25	4	1e	2g	7
Zhou et al^Citation23	4	1e	3	8
Cao et al^Citation20	4	1e	2g	7
Zhang et al^Citation40	0a–d	0e,f	1g,h	1
Luo et al^Citation15	4	0e,f	2g	6
Dhayat et al^Citation27	4	1f	3	8
Zhang et al^Citation26	4	1e	1g,i	6
Azizmohammadi et al^Citation18	4	1e	1g,h	6

Notes:

a Reasons for lost stars: no description of the derivation of the cohort;

b no description of the derivation of the nonexposed cohort;

c no description of exposure ascertainment;

d no description of whether outcome of interest was not present at the start of study;

e study not controlling the most important factor such as TNM stage;

f study not controlling other additional factors, such as age, gender, and smoke;

g no description of outcome assessment;

h inadequacy of follow-up of cohorts;

i follow-up not long enough for outcomes to occur.

Table 2 Characteristics of the included articles

Authors	Year	Origin	Cancer type	Treatment	Number of patients	Stage	miR-100 assay	Cutoff	Sample source	Follow-up (months)	Hazard ratio	Multivariate analysis
Feber et al^Citation12	2011	The USA	EA	Surgerya	45	I–IV	qRT-PCR	Median	Tissue	21.8c	K–M	No
Huang et al^Citation13	2012	People’s Republic of China	SCCC	Surgerya	42	I–IV	qRT-PCR	6.515b	Tissue	23.6d	K–M	No
Torres et al^Citation16	2012	Poland	EEC	Surgery + radiotherapy+ chemotherapy	73	I–IV	qRT-PCR	Median	Tissue	150e	Report	Yes
Peng et al^Citation17	2012	People’s Republic of China	EOC	Surgerya	98	I–IV	qRT-PCR	Median	Tissue	72e	Report	Yes
Wang et al^Citation19	2012	People’s Republic of China	BC	Surgery + chemotherapy	126	NR	qRT-PCR	Median	Tissue	36e	Report	Yes
Liu et al^Citation14	2012	People’s Republic of China	NSCLC	Surgerya	110	I–III	qRT-PCR	Mean	Tissue	60e	Report	Yes
Wang et al^Citation21	2013	People’s Republic of China	RCC	Surgerya	96	NR	qRT-PCR	Median	Tissue	81.8c	Report	Yes
Sun et al^Citation22	2013	People’s Republic of China	ESCC	Surgerya	61	I–III	qRT-PCR	NR	Tissue	120e	K–M	No
Chen et al^Citation24	2013	People’s Republic of China	HCC	Surgerya	134	I–IV	qRT-PCR	Mean	Tissue	60e	Report	Yes
Chen et al^Citation25	2014	People’s Republic of China	CRC	Surgerya	138	I–IV	qRT-PCR	Median	Tissue	60e	Report	Yes
Zhou et al^Citation23	2014	People’s Republic of China	ESCC	Radiotherapy	120	I–IV	qRT-PCR	Median	Tissue	60e	Report	Yes
Cao et al^Citation20	2015	People’s Republic of China	BC	Surgerya	92	NR	qRT-PCR	Median	Tissue	60e	Report	Yes
Luo et al^Citation15	2015	People’s Republic of China	NSCLC	Surgerya	48	NR	qRT-PCR	Median	Tissue	18e	K–M	No
Dhayat et al^Citation27	2015	Germany	PDA	Surgery + chemotherapy	91	II	qRT-PCR	5b	Tissue	30.5c	Report	Yes
Zhang et al^Citation26	2015	People’s Republic of China	CRC	Surgerya	172	I–IV	qRT-PCR	Median	Tissue	50e	Report	Yes
Azizmohammadi et al^Citation18	2016	Iran	EOC	NR	55	I–IV	qRT-PCR	Median	Tissue	40c	Report	Yes

Notes:

a No description of postoperative adjuvant therapy;

b did not state the definition of cutoff;

c median;

d mean;

e maximum duration of follow-up.

Abbreviations: BC, bladder cancer; CRC, colorectal cancer; EA, esophageal adenocarcinoma; EEC, endometrioid endometrial carcinoma; EOC, epithelial ovarian cancer; ESCC, esophageal squamous cell carcinoma; HCC, hepatocellular carcinoma; K–M, Kaplan–Meier curves; NR, not reported; NSCLC, non-small-cell lung cancer; PDA, pancreatic ductal adenocarcinoma; qRT-PCR, quantitative real-time polymerase chain reaction; RCC, renal cell carcinoma; ROC, receiver operating characteristic; SCCC, small cell carcinoma of the cervix.

Figure 2 Forest plot of the relationship between miR-100 and overall survival in solid tumor. Note: Weights are from random-effects analysis.

Abbreviations: CI, confidence interval; ES, effect size.

Table 3 Subgroup analysis

Subgroup	Heterogeneity		HR (95% CI)	P-value
	I^2	P-value
Patient origin
Asian	84.6%	<0.001	2.38 (1.55–3.66)a	<0.001
Non-Asian	90.0%	<0.001	0.60 (0.10–3.50)a	0.574
Cancer type
Non-small-cell lung cancer	0	0.696	2.46 (1.98–3.06)b	<0.001
Epithelial ovarian cancer	69.6%	0.070	2.29 (1.72–3.04)b	<0.001
Bladder cancer	52.7%	0.146	4.14 (1.85–9.27)b	0.001
Esophageal cancer	88.5%	<0.001	1.82 (0.32–10.32)a	0.497
Colorectal cancer	77.1%	0.037	1.7 (0.93–3.09)b	0.084
Multivariate analysis
Yes	88.0%	<0.001	1.91 (1.19–3.06)a	0.007
No	79.4%	0.002	2.03 (0.55–7.42)a	0.286

Notes:

a Random-effects model;

b fixed-effects model.

Abbreviations: CI, confidence interval; HR, hazard ratio.

Figure 3 Sensitivity analysis: meta-analysis of random-effects estimates (exponential form) with studies omitted.

Figure 4 Publication bias: Begg’s funnel plot with pseudo 95% confidence limits.

Abbreviations: HR, hazard ratio; SE, standard error.

FeberAXiLPennathurAMicroRNA prognostic signature for nodal metastases and survival in esophageal adenocarcinomaAnn Thorac Surg20119151523153021420070

 PubMed Web of Science ®Google Scholar

HuangLLinJXYuYHZhangMYWangHYZhengMDownregu-lation of six microRNAs is associated with advanced stage, lymph node metastasis and poor prognosis in small cell carcinoma of the cervixPLoS One201273e3376222438992

 PubMedGoogle Scholar

TorresATorresKPesciADeregulation of miR-100, miR-99a and miR-199b in tissues and plasma coexists with increased expression of mTOR kinase in endometrioid endometrial carcinomaBMC Cancer20121236922920721

 PubMed Web of Science ®Google Scholar

PengDXLuoMQiuLWHeYLWangXFPrognostic implications of microRNA-100 and its functional roles in human epithelial ovarian cancerOncol Rep20122741238124422246341

 PubMed Web of Science ®Google Scholar

WangSXueSDaiYReduced expression of microRNA-100 confers unfavorable prognosis in patients with bladder cancerDiagn Pathol2012715923173870

 PubMedGoogle Scholar

LiuJLuKHLiuZLSunMDeWWangZXMicroRNA-100 is a potential molecular marker of non-small cell lung cancer and functions as a tumor suppressor by targeting polo-like kinase 1BMC Cancer20121251923151088

 PubMedGoogle Scholar

WangGChenLMengJChenMZhuangLZhangLOverexpres-sion of microRNA-100 predicts an unfavorable prognosis in renal cell carcinomaInt Urol Nephrol201345237337923378187

 PubMed Web of Science ®Google Scholar

SunJChenZTanXMicroRNA-99a/100 promotes apoptosis by targeting mTOR in human esophageal squamous cell carcinomaMed Oncol201330141123292834

 PubMed Web of Science ®Google Scholar

ChenPZhaoXMaLDownregulation of microRNA-100 correlates with tumor progression and poor prognosis in hepatocellular carcinomaMol Cell Biochem20133831–2495823842624

 PubMed Web of Science ®Google Scholar

ChenPXiQWangQWeiPDownregulation of microRNA-100 correlates with tumor progression and poor prognosis in colorectal cancerMed Oncol2014311023525216869

 PubMed Web of Science ®Google Scholar

ZhouSYangBZhaoYXuSZhangHLiZPrognostic value of microRNA-100 in esophageal squamous cell carcinomaJ Surg Res2014192251552025218280

 PubMedGoogle Scholar

CaoYHZhangHHXuHFDuanYJLiQHuangBPrognostic role of microRNA-100 in patients with bladder cancerGenet Mol Res2015144159481595426662386

 PubMedGoogle Scholar

ZhangBZhaoRHeYMicroRNA 100 sensitizes luminal A breast cancer cells to paclitaxel treatment in part by targeting mTOROncotarget2016755702571426744318

 PubMedGoogle Scholar

LuoJChenBJiXXZhouSWZhengDOverexpression of miR-100 inhibits cancer growth, migration, and chemosensitivity in human NSCLC cells through fibroblast growth factor receptor 3Tumour Biol Epub2015828

 Google Scholar

DhayatSAAbdeenBKohlerGSenningerNHaierJMardinWAMicroRNA-100 and microRNA-21 as markers of survival and chemotherapy response in pancreatic ductal adenocarcinoma UICC stage IIClin Epigenetics2015713226705427

 PubMedGoogle Scholar

ZhangSYuanWTangWXuCMaJExpression of microRNA-100 and its relation with prognosis of colorectal cancerZhonghua Zhong Liu Za Zhi2015378603608 Chinese26714601

 PubMedGoogle Scholar

AzizmohammadiSAzizmohammadiSSafariAThe role and expression of miR-100 and miR-203 profile as prognostic markers in epithelial ovarian cancerAm J Transl Res2016852403241027347348

 PubMed Web of Science ®Google Scholar