Figures & data
Figure 1 MiR-99a is downregulated in NPC cell lines and clinical specimens.
Notes: (A) MiR-99a expression in NPC cell lines and the normal nasopharyngeal epithelial cell NP69. (B) MiR-99a expression in 4 paired NPC tissues and normal nasopharyngeal epithelial tissues. MiR-99a expression was normalized to U6 RNA. *P<0.05.
Abbreviation: NPC, nasopharyngeal carcinoma.
Abbreviation: NPC, nasopharyngeal carcinoma.
Figure 2 Overexpression of miR-99a inhibits nasopharyngeal carcinoma cell migration and invasion in vitro.
Notes: (A–C) Effect of miR-99a overexpression on cell migratory and invasive abilities was measured using wound healing assay (A), transwell migration (B), and invasion (C) assays after transfection of miR-99a mimics or miR control in CNE-2 and HONE-1 cells. *P<0.05.
Figure 3 Silencing of miR-99a promotes nasopharyngeal carcinoma cell migration and invasion in vitro.
Notes: (A–C) Effect of miR-99a silencing on cell migratory and invasive abilities was measured using wound healing assay (A), transwell migration (B), and invasion (C) assays after transfection of miR-99a inhibitor or control in CNE-2 and HONE-1 cells. *P<0.05.
Figure 4 Overexpression of miR-99a suppresses nasopharyngeal carcinoma lung metastasis in vivo.
Notes: (A–D) Lung metastatic colonization models were constructed by injecting HONE-1 cells stably overexpressing miR-99a into the tail vein of nude mice. Representative pictures (A) and quantification (B) of macroscopic metastatic tumor nodes formed on the lung surface. Representative pictures (C) and quantification (D) of microscopic metastatic tumor nodes formed in the lung. *P<0.05.
Figure 5 HOXA1 is a direct target of miR-99a in nasopharyngeal carcinoma.
Notes: (A) Wild type and mutant miR-99a target sequences of the 3′-UTR of HOXA1 mRNA; (B–D) HOXA1 mRNA (B) and protein (C) expression and luciferase activities (D) were measured by real-time RT-PCR, Western blotting, and luciferase reporter assays in CNE-2 and HONE-1 cells after miR-99a mimics or inhibitor transfection. *P<0.05.
Abbreviations: RT-PCR, reverse transcriptase polymerase chain reaction; 3′-UTR, 3′-untranslated region; CMV, cytomegalovirus; WT, wild-type; MT, mutant type.
Abbreviations: RT-PCR, reverse transcriptase polymerase chain reaction; 3′-UTR, 3′-untranslated region; CMV, cytomegalovirus; WT, wild-type; MT, mutant type.
Figure 6 HOXA1 is involved in miR-99a-mediated nasopharyngeal carcinoma cell migration and invasion.
Notes: (A–C) Effect of HOXA1 on cell migratory and invasive abilities was measured using wound healing assay (A), transwell migration (B), and invasion (C) assays after transfection of HOXA1 plasmid or vector in CNE-2 and HONE-1 cells pre-treated with miR-99a mimics. *P<0.05.
