Overexpression of ecotropic viral integration site-1 is a prognostic factor of lung squamous cell cancer

Figures & data

Figure 1 Representative images of high-expression and low-expression EVI1 detected with IHC.

Notes: (A) Representative image of low-expression EVI1 detected with IHC. EVI1 was mainly observed in the nucleus and, in some cells, it was expressed in cytoplasm. Scale bar: 50 μm. (B) Representative image of high-expression EVI1 detected with IHC. Scale bar: 50 μm.
Abbreviations: EVI1, ecotropic viral integration site-1; IHC, immunohistochemistry.

Table 1 Characteristics of patients with SCC

Characteristics	Number	Percentage
Gender
Male	106	64.63
Female	58	35.37
Age (years)
<60	47	28.66
≥60	117	71.34
Tumor diameter (cm)
≤3	61	37.20
>3	103	62.80
Differentiation
Well	85	51.83
Moderate	71	43.29
Poor	8	4.88
Lymph node metastasis
No (N0)	68	41.46
Yes (N1–3)	96	58.54
TNM stage
I	102	62.20
II	38	23.17
III	24	14.63
Smoking
No	76	46.34
Yes	88	53.66
EVI1
High	53	32.32
Low	111	67.68

Abbreviations: EVI1, ecotropic viral integration site-1; SCC, squamous cell cancer.

Table 2 Correlation between EVI1 expression and the clinicopathological parameters

Characteristics	EVI1		P-valuea
	Low	High
Gender			0.543
Male	70	36
Female	41	17
Age (years)			0.504
<60	30	17
≥60	81	36
Tumor diameter (cm)			0.391
≤3	44	17
>3	67	36
Differentiation			0.533
Poorly	59	26
Moderately	48	23
Well	4	4
Lymph node metastasis			0.738
No	45	23
Yes	66	30
TNM stage			0.616
I	68	34
II	28	10
III	15	9
Smoking			0.783
No	50	26
Yes	61	27

Note:

a χ² test.

Abbreviation: EVI1, ecotropic viral integration site-1.

Figure 2 Overall survival curves of the lymphatic status, pTNM stage, and EVI1 expression.

Notes: Overall survival curves of (A) positive and negative lymphatic invasion, (B) different pTNM stages, and (C) high- and low-expression EVI1 were analyzed by the Kaplan–Meier method, and the statistical difference was analyzed by the log-rank test.
Abbreviations: EVI1, ecotropic viral integration site-1; pTNM, pathological tumor-node-metastasis.

Table 3 Prognostic significance of EVI1 in SCC

Characteristics	Univariate analysis		Multivariate analysis
	5-year survival rate (%)	P-valuea	HR	95% CI	P-valueb
Gender		0.969			0.850
Male	25.4		1
Female	23.5		0.96	0.63–1.46
Age (years)		0.301			0.614
<60	22.6		1
≥60	25.3		1.13	0.71–1.79
Tumor diameter (cm)		0.896			0.970
≤3	25.5		1
>3	33.8		0.99	0.65–1.51
Differentiation		0.431			0.894
Well	15.6		1
Moderately + poorly	35.0		0.6	0.59–1.36
Lymph node metastasis		<0.001			<0.001
No	38.4		1
Yes	13.0		2.45	1.60–3.76
TNM stage		<0.001			–
I	32.4		–	–
II	22.5		–	–
III	0		–	–
Smoking		0.195			0.149
No	24.0		1
Yes	24.7		0.74	0.50–1.11
EVI1		0.021			0.013
Low	26.6		1
High	18.5		1.66	1.11–2.47

Notes:

a Log-rank test;

b Cox regression model. Bold figures denote P<0.05. ‘–’ indicates not enrolled in the multivariate analysis.

Abbreviations: CI, confidence interval; EVI1, ecotropic viral integration site-1; HR, hazard ratio; SCC, squamous cell cancer.