Apoptotic and genomic effects of corilagin on SKOV3 ovarian cancer cell line

Figures & data

Figure 1 Effects of corilagin on cell proliferation.

Notes: WST1 proliferation was performed using triplicate samples in three independent experiments. Statistical significance was determined using two-way analysis of variance, and P<0.05 was considered significant (*P<0.05; **P<0.01).

Figure 2 Effects of corilagin on caspase 3 enzymatic activity in SKOV3 cells.

Notes: Changes in caspase 3 enzymatic activity in response to increasing concentrations of corilagin in SKOV3 cells. The results are the means of two independent experiments. *P<0.05 was considered significant.

Figure 3 Effects of corilagin on membrane potential in SKOV3 cells.

Notes: Loss of mitochondrial membrane potential in response to increasing concentrations of corilagin in SKOV3 cells. The results are presented as the means of two independent experiments. *P<0.05 was considered significant.

Figure 4 Top networks and gene connections.

Notes: Literature-based clustering of differentially expressed genes using Ingenuity Pathway Analysis (IPA) after 78 hours of 100 µM corilagin stimulation in SKOV3 cells. According to IPA, three major pathways emerged: antigen processing and presentation, a MAPK signaling pathway, and a chemokine signaling pathway.

Table 1 Top networks and gene connections

KEGG ID	Title	Genes, n	Link to the KEGG map (multiple genes)
hsa04612	Antigen processing and presentation	12
hsa04010	MAPK signaling pathway	10
hsa05200	Pathways in cancer	8
hsa05206	Ovarian cancer	6
hsa04062	Chemokine signaling pathway	6

Notes: Literature-based clustering of differentially expressed genes using Ingenuity Pathway Analysis (IPA) after 78 hours of 100 µM corilagin stimulation in SKOV3 cells. According to IPA, three major pathways emerged: antigen processing and presentation, a MAPK signaling pathway, and a chemokine signaling pathway.

Abbreviation: KEGG, Kyoto Encyclopedia of Genes and Genomes.

Table 2 Genes involved in top network connections

Pathway name	Differentially expressed genes	Adjusted P-value
Adherens junction	EGFR, TCF7	9^−15
Antigen processing and presentation	CD8B, HLA-DQB1, HLA-DRA, HSPA6, IFNA6, KIR2DL5A, PDIA3	3.7^−14
Phosphatidylinositol signaling system	CDS1, DGKB, ITPR1, PIK3C2G, PRKCG	4.7^−6

Notes: Literature-based clustering of differentially expressed genes using Ingenuity Pathway Analysis (IPA) after 78 hours of 100 µM corilagin stimulation in SKOV3 cells. According to KEGG, three major pathways emerged: adherens junctions, antigen processing and presentation, and a phosphatidylinositol signaling system were the most statistically significant networks in response to corilagin treatment.

Abbreviation: KEGG, Kyoto Encyclopedia of Genes and Genomes.