RNA sequencing identifies gene expression profile changes associated with β-estradiol treatment in U2OS osteosarcoma cells

Figures & data

Table 1 Summary of clean reads alignment to the reference genome

Sample title	Treatment	Clean reads	Mapped reads	Map ratio (%)
14710C-1	Control	11660638	8129145	69.71
14710C-2	Control	12351701	8531536	69.07
14710C-3	E2	13820514	9485360	68.63
14710C-4	E2	11927581	7843445	65.76

Abbreviation: E2, estradiol.

Table 2 The top five GO terms of upregulated DEGs

Category	Term	Description	P-value	Genes
BP	GO:0010628	Positive regulation of gene expression	0.002272	NFKB1, ATF7IP, HDAC5, etc
BP	GO:0010629	Negative regulation of gene expression	0.002581	NFKB1, ATF7IP, HDAC5, etc
BP	GO:0016568	Chromatin modification	0.002959	HDAC5, MEN1, EPC1, etc
BP	GO:0045944	Positive regulation of transcription from RNA polymerase II promoter	0.004248	NFKB1, HDAC5, MEN1, etc
BP	GO:0016481	Negative regulation of transcription	0.004572	NFKB1, ATF7IP, HDAC5, etc

Abbreviations: BP, biological process; GO, gene ontology; DEGs, differentially expressed genes.

Table 3 The top five GO terms of downregulated DEGs

Category	Term	Description	P-value	Genes
BP	GO:0060070	Wnt receptor signaling pathway through β-catenin	0.002951	RARG, TBL1X, TCF7L2
BP	GO:0007409	Axonogenesis	0.01382	ALCAM, NRP1, SLC26A6, etc
BP	GO:0048667	Cell morphogenesis involved in neuron differentiation	0.01801	ALCAM, NRP1, SLC26A6, etc
BP	GO:0022402	Cell cycle process	0.01832	AKT1, DSN1, POLD1, etc
BP	GO:0048812	Neuron projection morphogenesis	0.01916	ALCAM, NRP1, SLC26A6, etc

Abbreviations: BP, biological process; GO, gene ontology; DEGs, differentially expressed genes.

Figure 1 PPI network of DEGs.

Note: Red nodes represent upregulated DEGs, green nodes represent downregulated DEGs, and blue nodes represent non-DEGs.
Abbreviations: PPI, protein–protein interaction; DEGs, differentially expressed genes.

Figure 2 Related NFKB1 (A) and AKT1 (B) expression levels in U2OS osteosarcoma cells treated with E2.

Note: **P<0.01.
Abbreviations: E2, estradiol; DMSO, dimethyl sulfoxide.