Circ-104916 is downregulated in gastric cancer and suppresses migration and invasion of gastric cancer cells

Figures & data

Figure 1 Circ-104916 expression in GC tissues.

Notes: (A) The expression of circ-104916 was validated by qPCR in seventy patients. Log2 fold changes in circ-104916 expressions (T/N) of each case are plotted. (B) The expression level of circ-104916 was significantly downregulated in tumor tissues. ^**P<0.01.
Abbreviation: qPCR, quantitative polymerase chain reaction.

Figure 2 Clinicopathological factors with the expression level of circ-104916.

Notes: (A) The expression of circ-104916 was much lower in the deeper invasion depth patients. (B) Circ-104916 expression was downregulated in the patients with more frequent lymphatic metastasis. (C) The expression of circ-104916 was lower in higher tumor-stage patients. ^**P<0.01.

Table 1 Correlation with the clinicopathological data and expression of circ-104916

Characteristics	Low	High	P-value
Total	35	35
Age (years)			0.339
<60	15	20
≥60	20	15
Sex			0.045
Male	27	18
Female	8	17
Differential status			0.785
Undifferentiated/poorly	25	27
Moderate/well	10	8
Nerve invasion			0.019
Yes	29	19
No	6	16
Vascular invasion			0.338
Yes	19	14
No	16	21
T stages			0.001
T1	1	12
T2–T4	34	23
N stage			0.019
N0	6	16
N1–N3	29	19
TNM stages			0.015
I–II	9	20
III–IV	26	15

Figure 3 Circ-104916 overexpression suppressed the proliferation of GC cells.

Notes: (A) Circ-104916 was more downregulated in GC cell lines (BGC823, MGC803, MKN45, SGC7901, AGS) than in the normal gastric mucosa cell line (GES-1). (B) The effects of the pcDNA-hsa-circ-104916 plasmid on the expression of circ-104916 in GC cell lines. (C) Effect of circ-104916 on cell proliferation in vitro by CCK-8 assay. Error bars represent mean ± SEM from three independent experiments. ^**P<0.01.
Abbreviations: GC, gastric cancer; CCK-8, Cell Counting Kit-8; OD, optical density; SEM, standard error of the mean.

Figure 4 Circ-104916 inhibited the migration and invasion of GC cells.

Notes: (A) After transfecting pcDNA-hsa-circ-0067934, GC cells showed reduced migration in transwell migration assays. (B) Circ-104916 also suppressed the invasion ability of BGC823 and MGC803 cells. ^**P<0.01. (C) After transfecting pcDNA-hsa-circ-104916, the protein level of key molecules of EMT was assessed by Western blot.
Abbreviations: GC, gastric cancer; EMT, epithelial–mesenchymal transition.

Figure S1 Hierarchical clustering analysis of the most up- and down regulated circRNAs.

Notes: Each column represents a sample, and each row represents a circRNA. Red strip represents a high relative expression, and green strip represents a low relative expression. T represents GC tissues and N represents normal gastric mucosa.

Abbreviation: GC, gastric cancer.