Evaluation of p53 gene expression and prognosis characteristics in uveal melanoma cases

Figures & data

Figure 1 Expressions of p53 mRNA in (A) MUM-2B, C918, and (B) D78 cells as detected by RT-PCR.

Abbreviations: mRNA, messenger RNA; RT-PCR, real-time polymerase chain reaction.

Table 1 RT-PCR primer sequence and silence sequence

Gene	Sequence
p53	Forward primer: 5′-TGCGTGTGGAGTATTTGGATG-3′
	Reverse primer: 5′-TGGTACAGTCAGAGCCAACCTC-3′
GAPDH	Forward primer: 5′-ATGGCACCGTCAAGGCTGAG-3′
	Reverse primer: 5′-GCAGTGATGGCATGGACTGT-3′
p53 siRNA	Sense sequence: 5′-CUACUUCCUGAAAACAACGdTdT-3′
	Antisense sequence: 5′-CGUUGUUUUCAGGAAGUAG [dT] [dT]-3′
NC siRNA	Sense sequence: 5′-ACUACUGAGUGACAGUAGA [dT] [dT]-3′
	Antisense sequence: 5′-UCUACUGUCACUCAGUAGU [dT] [dT]-3′

Abbreviations: RT-PCR, real-time polymerase chain reaction; siRNA, small interfering RNA; NC, negative control.

Figure 2 Expression of p53 protein in MUM-2B, C918, and D78 cells as detected by Western blot.

Figure 3 Expression of p53 protein in pathological tissues as detected by immunohistochemistry method (400×).

Figure 4 The cell invasion ability was measured by a transwell assay at 48 hours after p53 overexpression in cells.

Note: Magnification ×200.

Table 2 Expression of p53 protein in normal tissues and uveal melanoma tissues

Group	Case number	Positive	Negative	Positive expression rate (%)
Uveal melanoma tissues	88	68	20	77.27
Normal tissues	20	12	8	60

Table 3 Basic information of follow-up patients

p53	Case number	Death case number	Case number (survival)	%
Low expression	31	11	20	83.33
High expression	57	33	24	51.06
Total	88	44	44	57.35

Table 4 Comparison between p53 expression and the operational prognosis

	Chi-square	df	P-value*
Log rank (Mantel–Cox)	4.006	1	0.0453
Breslow (generalized Wilcoxon)	5.789	1	0.0161

Note:

* P<0.05 is considered statistically significance.