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Original Research

Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells

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Pages 4023-4028 | Published online: 11 Aug 2017

Figures & data

Figure 1 Quercetin inhibits U251 cell migration and invasion.

Notes: (A) Control and quercetin-treated confluent monolayer of U251 cells were scratched (0 hour) and allowed to migrate for 24 hours and 48 hours, respectively. (B) Transwell experiments showing control and quercetin-treated cells passing the membrane. (C) Number of cells passed the membrane for the control and quercetin-treated groups. *P<0.05. Magnification (A) ×100, (B) ×200.
Figure 1 Quercetin inhibits U251 cell migration and invasion.

Figure 2 Quercetin inhibits U251-induced endothelial cell invasion and tube formation.

Notes: (A) Transwell experiments showing the control and quercetin-treated cells passing to the bottom wells, where U251-conditioned medium was applied. (B) Quantification of cell numbers for the control and quercetin-treated groups. (C) Endothelial cell tube formation in the presence of control and 10 μg/mL quercetin. (D) Quantification of tube formation for the control and quercetin-treated groups. *P<0.05.
Figure 2 Quercetin inhibits U251-induced endothelial cell invasion and tube formation.

Figure 3 Quercetin inhibits VEGFA, MMP9, and MMP2 expression.

Note: Immunoblot showing protein levels of VEGFA, MMP9, and MMP2 in U251 cells in response to 0 μg/mL (control) and 10 μg/mL quercetin.
Figure 3 Quercetin inhibits VEGFA, MMP9, and MMP2 expression.