The effect of circulating miR-223 on surveillance of different cancers: a meta-analysis

Figures & data

Figure 1 Flow of the studies in the meta-analysis.

Table 1 Basic data for all included studies in the meta-analysis

References	Year	Country	Sample size	Cancer-type	Method	Follow-up (months)	Cutoff (high/low)	TNM stage		Survival		Quality score
								Low	High	Method	Outcomes
Sanfiorenzo et al^Citation24	2013	France	52	NSCLC	qrt-PCR	60	24/28	NR	NR	Multivariate	DFS	8
Heegaard et al^Citation15	2012	USA	180	NSCLC	qrt-PCR	60	90/90	NR	NR	Univariate	OS	8
Wu et al^Citation17,^*	2014	China	194	ESCC	qrt-PCR	60	113/81	1:3	1:5	Multivariate	OS	8
								2:48	2:62
								3:21	3:36
								4:5	4:2
Wu et al^Citation18	2014	China	63	ESCC	qrt-PCR	24	NR	NR	NR	Univariate	OS	8
Komatsu et al^Citation19	2015	Japan	46	PC	qrt-PCR	50	25/20	NR	NR	Univariate	CSS	8
Stamatopoulos et al^Citation20	2009	Belgium	110	CLL	qrt-PCR	300	37/73	NR	NR	Univariate	OS, TFS	8
Zhou et al^Citation13	2012	China	53	CLL	qrt-PCR	90	31/22	NR	NR	Univariate	OS, TFS	8
Dong et al^Citation21	2016	China	112	OSC	qrt-PCR	60	59/53	2a:22	2a:50	Multivariate	OS	8
								2b/3:40	2b/3:10
Wu et al^Citation22	2014	China	94	NSCLC	qrt-PCR	48	47/47	3:23	3:29	Multivariate	OS	8
								4:24	4:18
Qu^Citation23	2014	China	98	MM	qrt-PCR	48	49/49	1+2:22	1+2:30#	Univariate	OS, PFS	8
								3:27	3:19

Note:

# ISS, international staging system.

Abbreviations: NSCLC, nonsmall cell lung carcinoma; ESCC, esophageal squamous cell carcinoma; PC, pancreatic cancer; CLL, chronic lymphocytic leukemia; OSC, osteosarcoma; OS, overall survival; MM, multiple myeloma; TNM, tumor node metastasis; qrt-PCR, quantitative real-time PCR; DFS, disease-free survival; TFS, treatment-free survival; CSS, cause-specific survival; PFS, progression-free survival; NR, not reported.

Figure 2 Forest plot of association between circulating miR-223 expression and TNM.

Abbreviations: CI, confidence interval; M–H, Mantel–Haenszel test; TNM, tumor node metastasis.

Figure 3 Forest plot showing the association between circulating miR-223 expression and OS. (A) Pooled HR of circulating miR-223 on OS of all types of cancer under random-effects model. In subgroup analyses, (B) pooled HR of circulating miR-223 on OS of chronic lymphocytic leukemia under the fixed-effects model; (C) pooled HR of circulating miR-223 on OS of nonsmall cell lung carcinoma under fixed-effects model; (D) pooled HR of circulating miR-223 on OS of esophageal squamous cell carcinoma under the random-effects model. Data are HRs and 95% CIs.

Abbreviations: SE, standard error; IV, inverse variance; CI, confidence interval; OS, overall survival; HR, hazard ratio.

Figure 4 Forest plot of association between circulating miR-223 expression and TFS.

Abbreviations: CI, confidence interval; IV, inverse variance; SE, standard error; TFS, treatment-free survival.

Table 2 Sensitivity analysis for the studies that resulted in loss of significance after omission

Original summary effects				Omitted study	Resulting summary effects
HR	95% CI	Nature of association	I^2		HR	95% CI	Nature of association	I^2
0.64	0.38–1.11	Not significantly decreased risk	76%	Heegaard et al^Citation15	0.59	0.29–1.21	Not significantly decreased risk	78%
				Wu et al^Citation17	0.54	0.33–0.89	Significantly decreased risk	57%
				Wu et al^Citation18	0.57	0.29–1.13	Not significantly decreased risk	79%
				Stamatopoulos et al^Citation20	0.77	0.46–1.29	Not significantly decreased risk	71%
				Zhou et al^Citation13	0.66	0.38–1.13	Significantly increased risk	78%
				Dong et al^Citation21	0.79	0.48–1.30	Not significantly decreased risk	69%
				Wu et al^Citation22	0.61	0.33–1.13	Not significantly decreased risk	79%
				Qu^Citation23	0.65	0.37–1.14	Not significantly decreased risk	79%

Abbreviations: CI, confidence interval; HR, hazard ratio.

Figure 5 Begg’s funnel plot of the association between circulating miR-223 and OS in all cancer types under random-effects model.

Abbreviations: OS, overall survival; SE, standard error.

Figure S1 Forest plot of the pooled HRs for OS with esophageal squamous cell carcinoma excluded under the random-effects model.

Abbreviations: HR, hazard ratio; OS, overall survival; IV, inverse variance; CI, confidence interval; SE, standard error.

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