Figures & data
Notes: (A) A microarray containing 8,277 lncRNA probes was used to identify differentially expressed lncRNAs in three PCa patients. (B) Relative LOC440040 expression level in PCa tissues compared with paired adjacent normal tissues. (C) Relative LOC440040 expression level in two PCa cell lines (PC-3, 22RV1) and normal prostate cell line (WPMY-1). *P<0.05.
Abbreviations: lncRNA, long noncoding RNA; PCa, prostate cancer.
Abbreviations: lncRNA, long noncoding RNA; PCa, prostate cancer.
Note: Patients in the high LOC440040 expression group exhibited significantly poorer prognosis than those in the low LOC440040 expression group.
Abbreviation: PCa, prostate cancer.
Abbreviation: PCa, prostate cancer.
Notes: (A and B) Expression of LOC440040 in PC-3 and 22RV1 cells was significantly downregulated after si-LOC440040 transfection. (C and D) Cell proliferation was measured by CCK-8 assay in PC-3 and 22RV1 cells transfected with si-LOC440040 or si-NC. *P<0.05.
Abbreviations: CCK-8, Cell Counting Kit-8; OD, optical density; PCa, prostate cancer; si-NC, negative control siRNA; siRNA, short interfering RNA.
Abbreviations: CCK-8, Cell Counting Kit-8; OD, optical density; PCa, prostate cancer; si-NC, negative control siRNA; siRNA, short interfering RNA.
Notes: (A and B) Colony formation assay was performed to determine the proliferation of PC-3 and 22RV1 cells after si-LOC440040 transfection. (C–G) Western blot protein expression levels in PC-3 and 22RV1 cells after si-LOC440040 transfection. GAPDH protein was used as an internal control. *P<0.05.
Abbreviations: PCa, prostate cancer; si-NC, negative control siRNA; siRNA, short interfering RNA.
Abbreviations: PCa, prostate cancer; si-NC, negative control siRNA; siRNA, short interfering RNA.
Notes: (A and B) The Transwell migration assay showed that the number of migrated cells was significantly lower in the si-LOC440040-transfected group than in the si-NC-transfected group. (C and D) The Transwell invasion assay showed that the number of invaded cells was significantly lower in the si-LOC440040-transfected group than in the si-NC-transfected group. (E–G) Western blot protein expression levels in PC-3 and 22RV1 cells after si-LOC440040 transfection. GAPDH protein was used as an internal control. *P<0.05.
Abbreviations: PCa, prostate cancer; si-NC, negative control siRNA; siRNA, short interfering RNA.
Abbreviations: PCa, prostate cancer; si-NC, negative control siRNA; siRNA, short interfering RNA.