IGF2BP3 as a potential tissue marker for the diagnosis of esophageal high-grade intraepithelial neoplasia

Figures & data

Table 1 The clinicopathological features of enrolled patients

Characteristics	N=112
Gender
Male	68
Female	44
Age (years), range; mean ± SD	37–82; 61.28±10.76
Location
Upper	32
Middle	31
Lower	49
Length
<5 cm	82
>5 cm	30
Circumferential extent
<1/2	30
1/2–2/3	46
>2/3	36
pT stage
pT1a-EP	21
pT1a-LPM	38
pT1a-MM	53

Notes: pT stage: pathological T stage; pT1a-EP: M1, carcinoma in situ; pT1a-LPM: M2, tumor-invaded lamina propria mucosa; pT1a-MM: M3, tumor invaded the whole layer of mucosa.

Figure 1 Analyses of IGF2BP3 expression in HGIN by Western blotting.

Notes: A total of 12 samples were analyzed. According to the final pathological diagnosis of 12 samples, the first, second, and fifth were M1; the third, sixth, seventh, and ninth were M2; and the remaining were M3. Seven cases showed moderate to strong IGF2BP3 expression, and five cases showed negative to weak expression. The relative expression of proteins was normalized to GAPDH.
Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HGIN, high-grade intraepithelial neoplasia; IGF2BP3, insulin-like growth factor-II mRNA-binding protein-3.

Figure 2 Analysis of IGF2BP3 expression in HGIN by IHC.

Notes: All images were captured at 200× magnification. Positive (A) and negative (B) staining of IGF2BP3 in pT1a-EP (M1). Positive (C) and negative (D) staining of IGF2BP3 in pT1a-LPM (M2). Positive (E) and negative (F) staining of IGF2BP3 in pT1a-MM (M3). Positive staining of IGF2BP3 was characterized by a dark brown staining in the cytoplasm of the neoplastic cells.
Abbreviations: HGIN, high-grade intraepithelial neoplasia; IGF2BP3, insulin-like growth factor-II mRNA-binding protein-3; IHC, immunohistochemistry.

Table 2 Immunohistochemical intensity data on IGF2BP3 expression

pT stage group	N	Intensity				P-value
		Negative	Weak	Moderate	Strong
pT stage	112	44	20	24	24	0.038
pT1a-EP	21	13	5	3	0
pT1a-LPM	38	15	8	8	7
pT1a-MM	53	16	7	13	17

Notes: pT stage: pathological T stage; pT1a-EP: M1, carcinoma in situ; pT1a-LPM: M2, tumor-invaded lamina propria mucosa; pT1a-MM: M3, tumor invaded the whole layer of mucosa.

Table 3 Correlation between IGF2BP3 expression and the patient demographics and endoscopic findings in HGIN

Characteristics	IGF2BP3 expression		P-value
	Positive (n=68)	Negative (n=44)
Gender			0.282
Male	44	24
Female	24	20
Age (years)			0.754
≤50	14	8
>50	54	36
Location			0.294
Upper	16	16
Middle	19	12
Lower	33	16
Length			0.731
≤5 cm	49	33
>5 cm	19	11
Circumferential extent			0.370
<1/2	15	15
1/2–2/3	30	16
>2/3	23	13
pT stage			0.042
pT1a-EP	8	13
pT1a-LPM	23	15
pT1a-MM	37	16

Notes: pT stage: pathological T stage; pT1a-EP: M1, carcinoma in situ; pT1a-LPM: M2, tumor-invaded lamina propria mucosa; pT1a-MM: M3, tumor invaded the whole layer of mucosa.

Abbreviations: HGIN, high-grade intraepithelial neoplasia; IGF2BP3, insulin-like growth factor-II mRNA-binding protein-3.

Table 4 Univariate and multiple linear regression analysis for the correlations between the positive expression of IGF2BP3 and predictors

Predictors	Univariate			Multiple
	β	Wald	P-value	β	Wald	P-value
Gender (male/female)	0.411	0.967	0.326	0.429	0.991	0.319
Age (≤50/>50 years)	−0.11	0.000	0.983	0.021	0.001	0.969
Location (upper/middle/lower)	−0.329	1.340	0.247	−0.635	1.281	0.258
Length (≤5 cm/>5 cm)	0.133	0.073	0.787	0.091	0.034	0.854
Circumferential extent (<1/2/1/2–2/3/>2/3)	−0.102	0.133	0.715	−0.023	0.002	0.969
pT (pT1a-EP/pT1a-LPM/pT1a-MM)	−0.647	5.508	0.019	−1.343	5.257	0.022

Notes: pT: pathological T; pT1a-EP: M1, carcinoma in situ; pT1a-LPM: M2, tumor-invaded lamina propria mucosa; pT1a-MM: M3, tumor invaded the whole layer of mucosa.

Abbreviation: IGF2BP3, insulin-like growth factor-II mRNA-binding protein-3.