Figures & data
Figure 1 HOXD-AS1 is overexpressed in NSCLC and correlates with patient outcome.
Abbreviations: HOXD-AS1, HOXD cluster antisense RNA 1; NCTs, noncancerous tissues; NSCLC, non-small cell lung cancer; qRT-PCR, quantitative reverse-transcription polymerase chain reaction.
![Figure 1 HOXD-AS1 is overexpressed in NSCLC and correlates with patient outcome.](/cms/asset/a170dd7e-be01-4048-9f15-aa71ae618ffb/dott_a_12195665_f0001_b.jpg)
Table 1 Correlation of the expression of HOXD-AS1 with NSCLC clinical pathologic features
Figure 2 The expression of HOXD-AS1 affects NSCLC cell proliferation, cell cycle and apoptosis.
Abbreviations: CTRL, control; HOXD-AS1, HOXD cluster antisense RNA 1; NC, negative control; NSCLC, non-small cell lung cancer; qRT-PCR, quantitative reverse-transcription polymerase chain reaction; FL, fluorescence; PI, propidium iodide.
![Figure 2 The expression of HOXD-AS1 affects NSCLC cell proliferation, cell cycle and apoptosis.](/cms/asset/96d2e400-1f2a-4ade-b4e1-228265ab9114/dott_a_12195665_f0002_b.jpg)
Figure 3 HOXD-AS1 negatively regulates the expression of miR-147a in NSCLC cells.
Abbreviations: HOXD-AS1, HOXD cluster antisense RNA 1; HOXD-AS1-MUT, HOXD-AS1 sequence with mutational miR-147a binding site, which was cloned into pGL3 vector; HOXD-AS1-WT, HOXD-AS1 sequence with miR-147a wild-type binding site, which was cloned into pGL3 vector; NC, negative control; NSCLC, non-small cell lung cancer.
![Figure 3 HOXD-AS1 negatively regulates the expression of miR-147a in NSCLC cells.](/cms/asset/d0c60428-a916-4fde-aae0-17674414b5ae/dott_a_12195665_f0003_b.jpg)
Figure 4 miR-147a inhibition abrogates the effect of HOXD-AS1 knockdown on NSCLC cell proliferation by regulating pRB expression.
Abbreviations: CCK-8, cell counting kit-8; HOXD-AS1, HOXD cluster antisense RNA 1; NC, negative control; NSCLC, non-small cell lung cancer; pRB, retinoblastoma protein; FL, fluorescence; PI, propidium iodide.
![Figure 4 miR-147a inhibition abrogates the effect of HOXD-AS1 knockdown on NSCLC cell proliferation by regulating pRB expression.](/cms/asset/e974de9f-25d5-400e-8554-6c81a46c4a15/dott_a_12195665_f0004_b.jpg)
![Figure 4 miR-147a inhibition abrogates the effect of HOXD-AS1 knockdown on NSCLC cell proliferation by regulating pRB expression.](/cms/asset/b9cf94a0-181b-435c-aeb2-3749aa37ee0a/dott_a_12195665_f0004a_b.jpg)