Targeted inhibition of the phosphoinositide 3-kinase impairs cell proliferation, survival, and invasion in colon cancer

Figures & data

Figure 1 BEZ235 inhibited cell proliferation of HT-29 and HCT-116 cells.

Notes: (A) Western blotting results showed that BEZ235 inhibited p-Akt expression, and 1 μM BEZ235 had the most inhibition. (B) Western blotting results demonstrated that BEZ235 inhibited the activation of Akt and mTOR. (C) Cell Counting Kit-8 assay showed that BEZ235 significantly inhibited HT-29 and HCT-116 cell proliferation at 24, 48, and 72 hours. (D) Colony formation assays showed that BEZ235 inhibited the colony formation of HT-29 cells. (E) BEZ235 inhibited colony formation of HCT-116 cells. *P<0.05 and **P<0.001 versus control.
Abbreviations: OD450, optical density 450 nm; NC, negative control.

Figure 2 PI3KCA knockdown inhibited proliferation of HT-29 and HCT-116 cells.

Notes: (A) RT-PCR result showed that the expression of PI3KCA mRNA was significantly inhibited after shRNA transfection. (B) Western blotting results showed that shRNA transfection inhibited the activation of Akt and mTOR. (C) The quantitative analysis of Western blotting result. (D) Cell Counting Kit-8 assay showed that PI3KCA knockdown significantly inhibited the proliferation of HT-29 and HCT-116 cells. (E) Colony formation assays showed that colony formation was inhibited in PI3KCA-knockdown HT-29 cells. (F) PI3KCA knockdown inhibited the colony formation of HCT-116 cells. *P<0.05 and **P<0.001 versus control.
Abbreviations: OD450, optical density 450 nm; NC, negative control.

Figure 3 Inhibition of PI3K/Akt/mTOR pathway by BEZ235 and PI3KCA knockdown resulted in decreased cell migration and invasion.

Notes: (A) Transwell assay showed that cell migration of HT-29 and HCT-116 were obviously inhibited by BEZ235 and PI3KCA knockdown. (B) Cell invasion of HT-29 and HCT-116 were significantly inhibited by BEZ235 and PI3KCA knockdown. *P<0.05 versus control.
Abbreviation: NC, negative control.

Figure 4 Inhibition of PI3K/Akt/mTOR pathway by BEZ235 and PI3KCA knockdown induced cell apoptosis.

Notes: (A) Flow cytometry assay showed that cell apoptosis of HT-29 and HCT-116 were induced by BEZ235 and PI3KCA knockdown. (B) Western blotting results showed that BEZ235 treatment and PI3KCA knockdown inhibited Bcl-2 and Bim expression and induced cleavage caspase 3 and Bax expression. *P<0.05 versus controls.
Abbreviations: FITC, fluorescein isothiocyanate; NC, negative control; PI, propidium iodide.