The expression profile and clinical significance of circRNA0003906 in colorectal cancer

Figures & data

Figure 1 Relative circRNA0003906 expression in CRC cell lines.

Notes: circRNA0003906 expression levels in six CRC cell lines (HCT8, HCT116, SW480, SW620, HT29, and LoVo) and normal colon epithelial cell line NCM460 was determined by qRT-PCR. The values are given as mean ± SD of three independent experiments. *P<0.05.
Abbreviations: CRC, colorectal cancer; qRT-PCR, quantitative real-time polymerase chain reaction; SD, standard deviation.

Figure 2 Relative circRNA0003906 expression in CRC tissues.

Notes: (A) Of 122 CRC patients, 92 (75.4%) exhibited significant downregulation of circRNA0003906 in cancerous tissues compared with adjacent nontumor tissues. (B) circRNA0003906 expression level in 122 CRC tissues compared with adjacent nontumor tissues. The values are mean ± SD of three independent experiments. **P<0.0001.
Abbreviations: CRC, colorectal cancer; SD, standard deviation.

Table 1 Clinicopathological correlation of circRNA0003906 expression in CRC

Variables	Tumor circRNA0003906 expression		P-valuea
	Low	High
Age			0.419
<60 years	19	15
≥60 years	42	46
Gender			0.308
Male	47	42
Female	14	19
Tumor diameter			0.560
<5 cm	40	43
≥5 cm	21	18
Differentiation			0.026
Well + moderate	31	43
Poor	30	18
T classification			0.147
1–2	25	33
3–4	36	28
N classification			<0.001
0	21	46
1–2	40	15
TNM stage			0.146
I–II	29	37
III–IV	32	24
CEA			0.389
Negative	45	49
Positive	16	12
CA199			0.783
Negative	54	53
Positive	7	8

Notes: CRC patients receiving radical operation were segregated into circRNA0003906 high/low expression groups (calculation of cut-off by ROC analysis).

a Statistical analyses were performed with χ² test or Fisher’s exact test.

Abbreviations: CA199, carbohydrate antigen 19-9; CEA, carcinoembryonic antigen; CRC, colorectal cancer; ROC, receiver operating characteristics.

Figure 3 The ROC curve for differentiating CRC tissues from healthy controls.

Notes: The ROC curve was constructed using IBM SPSS Statistics for Windows, Version 22.0 (IBM Corp., Armonk, NY, USA). The area under the ROC curve was 0.818 (95% confidence interval =0.749–0.888; P<0.001). The specificity and sensitivity were 0.725 and 0.803, respectively.
Abbreviations: CRC, colorectal cancer; ROC, receiver operating characteristics.

Figure S1 The melt curve of circRNA0003906.