LncRNA expression and implication in osteosarcoma: a systematic review and meta-analysis

Figures & data

Table 1 Inclusion criteria for studies on the prognosis of osteo-sarcoma based on lncRNA expression levels

Inclusion items	Selection criteria
Tumor type	Osteosarcoma
Sample type	Tumor tissue or blood
Assay method	qRT-PCR or FISH
Time of study	January 2003 to September 2017
Follow-up (months)	≥60
Included results	Multivariate analysis of OS and Kaplan–Meier survival curves

Abbreviations: FISH, fluorescence in situ hybridization; lncRNA, long noncoding RNA; OS, overall survival; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction.

Figure 1 The flowchart of the selection process.

Abbreviation: OS, overall survival.

Table 2 Basic information of included articles

Reference	Year	LncRNA	Total patients (n)	Survival analysis	Follow-up (months)
Wang et al^Citation21	2017	TUG1	44	OS	120
Wen et al^Citation23	2017	UCA1	151	OS	60
Zhou et al^Citation30	2017	CCAL	46	OS	60
Chen et al^Citation24	2016	BCAR4	60	OS	60
Cong et al^Citation32	2016	TUSC7	82	OS	120
Gao and Lian^Citation27	2016	MALAT1	162	OS	65
Ju et al^Citation25	2016	BCAR4	168	OS	68
Li et al^Citation26	2016	HIF2PUT	82	OS	60
Li et al^Citation22	2016	UCA1	135	OS	60
Ma et al^Citation20	2016	TUG1	76	OS	60
Uzan et al^Citation33	2016	HULC	33	OS	96
Xia et al^Citation29	2016	91H	67	OS	60
Zhao et al^Citation15	2016	HNF1A-AS1	43	OS	60
Li et al^Citation28	2015	HOTTIP	68	OS	60
Sun et al^Citation17	2015	HULC	78	OS	60
Tian et al^Citation31	2015	MEG3	64	OS	60

Abbreviations: lncRNA, long noncoding RNA; OS, overall survival.

Table 3 HRs of lncRNA expression in osteosarcoma

LncRNA	HR (OS)	95% CI		P-value	lnHR	SE	Related to poor prognosis
		Lower	Upper
TUG1	2.78	1.29	6.00	0.009	1.02	0.39	High
TUG1	3.6	1.01	12.8	0.035	1.28	0.65	High
UCA1	2.52	1.32	4.83	0.011	0.92	0.33	High
UCA1	3.13	1.29	7.55	0.015	1.14	0.45	High
BCAR4	3.22	0.89	7.88	0.014	1.17	0.55	High
BCAR4	2.32	1.24	5.62	0.018	0.84	0.40	High
HULC	2.27	0.61	8.44	0.016	0.82	0.67	High
HULC	2.28	1.48	5.43	0.009	0.83	0.33	High
HIF2PUT	5.48	1.99	12.29	0.01	1.70	0.46	High
MALAT1	3.16	1.56	6.88	0.003	1.15	0.38	High
HNF1A-AS1	2.64	1.39	7.42	0.005	0.97	0.43	High
HOTTIP	2.89	1.37	7.06	0.007	1.06	0.42	High
91H	3.13	1.32	7.49	0.010	1.04	0.44	High
CCAL	3.18	1.64	9.94	0.021	1.16	0.46	High
MEG3	0.81	0.27	0.97	0.006	−0.21	0.32	Low
TUSC7	0.31	0.09	0.87	0.020	−1.17	0.58	Low

Abbreviations: CI, confidence interval; HR, hazard ratio; lncRNA, long noncoding RNA; lnHR, log hazard ratio; OS, overall survival; SE, standard error.

Figure 2 Forest plots of studies evaluating HRs of high TUG1 expression as compared to low expression.

Abbreviations: CI, confidence interval; HR, hazard ratio; IV, inverse variance; SE, standard error.

Figure 3 Forest plots of studies evaluating HRs of high UCA1 expression as compared to low expression.

Abbreviations: CI, confidence interval; HR, hazard ratio; IV, inverse variance; SE, standard error.

Figure 4 Forest plots of studies evaluating HRs of high BCAR4 expression as compared to low expression.

Abbreviations: CI, confidence interval; HR, hazard ratio; IV, inverse variance; SE, standard error.

Figure 5 Forest plots of studies evaluating HRs of high HULC expression as compared to low expression.

Abbreviations: CI, confidence interval; HR, hazard ratio; IV, inverse variance; SE, standard error.

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