B7-H3 promotes metastasis, proliferation, and epithelial-mesenchymal transition in lung adenocarcinoma

Figures & data

Table 1 B7-H3 expression and clinicopathological features of patients with lung adenocarcinoma

Features	n	B7-H3 expression		P-value
		Low expression	High expression
Age (years)				>0.05
≥60	76	33	43
<60	75	35	40
Gender				>0.05
Male	83	33	50
Female	68	35	33
Smoking				>0.05
Yes	78	35	43
No	73	33	40
Differentiation				>0.05
Well/moderate	62	25	37
Poor	89	43	46
Tumor size				>0.05
≤3 cm	28	10	18
>3 cm	123	58	65
Lymph node metastasis				<0.05
Yes	104	35	69
No	47	33	14
TNM stage				<0.05
I	21	17	4
II	42	23	19
III	38	13	25
IV	50	15	35
EGFR gene mutation				>0.05
Yes	72	31	41
No	47	22	25
Unknown	32	15	17

Figure 1 The B7-H3 expression in lung adenocarcinoma and paracancerous tissues.

Notes: (A) Low expression of B7-H3 in paracancerous tissues; (B) high expression of B7-H3 in paracancerous tissues; (C) low expression of B7-H3 in lung adenocarcinoma tissues; (D) high expression of B7-H3 in lung adenocarcinoma tissues; (E) Western blot analysis of B7-H3 expression levels in lung adenocarcinoma (T) and normal tissues (N); (F) B7-H3 expression and overall survival (OS).

Table 2 Univariate and multivariate analysis of overall survival

Clinical parameters	Univariate analysis		Multivariate analysis
	HR (95% CI)	P-value	HR (95% CI)	P-value
Age	0.781 (0.472–1.291)	0.816
Gender	0.932 (1.351–4.774)	0.055
Smoking	1.047 (0.710–1.546)	0.335
Differentiation	1.080 (0.695–1.680)	0.731
EGFR gene mutation	0.666 (0.411–1.081)	0.200
Lymph node metastasis	1.907 (1.136–5.398)	0.023
Tumor size	0.581 (0.294–1.065)	0.077
TNM stage	2.292 (1.498–3.331)	0.000	2.077 (1.389–3.141)	0.000
B7-H3 expression	3.167 (1.616–6.327)	0.001	3.187 (1.601–6.310)	0.000

Abbreviation: HR, hazard ratio.

Figure 2 (A) The transfection efficiencies observed using fluorescence microscopy; (B) B7-H3 expression levels detected in A549 cell lines by Western blot.

Note: **P<0.01.

Figure 3 Silencing B7-H3 reduces proliferation, invasion and migration of NSCLC cells.

Notes: (A) Viability measured by MTT assay in A549 cells; (B) transwell invasion and migration assay with control and B7-H3 siRNA-transfected A549 cells. *P<0.05; **P<0.01.
Abbreviation: NSCLC, non-small cell lung carcinoma.

Figure 4 B7-H3 regulates the protein expression levels of E-cadherin, N-cadherin and vimentin.