Figures & data
Figure 1 The expression of miR-300 in OSCC tissues and cells.
Notes: (A) The expression of miR-300 was compared between OSCC tissues and normal tissues. (B) The expression of miR-300 was compared between OSCC patients with metastasis and those without metastasis. (C) The expression of miR-300 was compared between two OSCC cell lines and the HaCat cells. *P<0.05.
Abbreviation: OSCC, oral squamous cell carcinoma.
Abbreviation: OSCC, oral squamous cell carcinoma.
Table 1 The relationship between miR-300 and the clinicopathologic characteristics in OSCC patients
Table 2 Univariate and multivariate Cox proportional hazards model for OS and DFS in OSCC patients
Figure 2 The prognostic value of miR-300 in OSCC.
Note: Kaplan–Meier plots were used to compare the (A) overall survival rate and (B) disease-free survival rate between patients with high level of miR-300 and those with low miR-300 level.
Abbreviation: OSCC, oral squamous cell carcinoma.
Abbreviation: OSCC, oral squamous cell carcinoma.
Figure 3 miR-300 inhibits the proliferation and invasion abilities.
Notes: (A, C) Tca8113 and Cal-27 cells were transfected with miR-300 mimics and inhibitor for 48 hours. The levels of miR-300 were determined by reverse transcription quantitative PCR assay. (B, D) The cells were treated as indicated above, and the cell proliferation ability was determined by MTS assay. (E, F) The cells were treated as indicated above, and the cell invasion ability was determined by Transwell. *P<0.05, **P<0.01.
Abbreviations: PCR, polymerase chain reaction; NC, negative control.
Abbreviations: PCR, polymerase chain reaction; NC, negative control.
Figure 4 miR-300 inhibits the EMT of OSCC cells.
Notes: (A, C) miR-300 overexpression significantly increased the mRNA level of E-cadherin and decreased the mRNA level of N-cadherin, Vimentin, Snail1 and MMP-2 in OSCC cells. (B, D) miR-300 overexpression significantly increased the protein expression of E-cadherin and decreased the expression of N-cadherin, Vimentin, Snail1 and MMP-2 in OSCC cells. (E, G) miR-300 downregulation significantly decreased the mRNA level of E-cadherin and increased the mRNA level of N-cadherin, Vimentin, Snail1 and MMP-2 in OSCC cells. (F, H) miR-300 downregulation significantly decreased the protein expression of E-cadherin and increased the expression of N-cadherin, Vimentin, Snail1 and MMP-2 in OSCC cells. *P<0.05.
Abbreviations: EMT, epithelial–mesenchymal transition; OSCC, oral squamous cell carcinoma; NC, negative control.
Abbreviations: EMT, epithelial–mesenchymal transition; OSCC, oral squamous cell carcinoma; NC, negative control.
Figure 5 ET-1 promotes cell migration and EMT expression by downregulating miR-300 expression in OSCC cells.
Notes: (A) Cells were incubated with ET-1 (10–100 nM) for 24 hours, and miR-300 expression was examined by q-PCR. Cells were transfected with a control miRNA or miR-300 mimic for 24 hours and then stimulated with ET-1 (100 nM) for 24 hours. (B) Invasion and (C, D) EMT marker expression were examined by Transwell invasion assay, RT-qPCR and Western blot. *P<0.05.
Abbreviations: EMT, epithelial–mesenchymal transition; ET-1, endothelin-1; OSCC, oral squamous cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
Abbreviations: EMT, epithelial–mesenchymal transition; ET-1, endothelin-1; OSCC, oral squamous cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
