Circ-DLG1 promotes the proliferation of esophageal squamous cell carcinoma

Figures & data

Figure 1 (A) Schematics showed that circ-DLG1 was derived from exons 13 to 18 of DLG1. (B) qRT-PCR for the abundance of circ-DLG1 and DLG1 mRNA in ESCC cells treated with RNase R. (C) qRT-PCR for the abundance of circ-DLG1 and DLG1 mRNA in ESCC cells treated with actinomycin.

Abbreviations: ESCC, esophageal squamous cell carcinoma; qRT-PCR, quantitative reverse transcription-polymerase chain reaction.

Table 1 The relationship between circ-DLG1 expression levels (−ΔCt) in gastric cancer plasmas and clinicopathologic factors of patients

Variables	No of patients (%)	Mean ± SE	P-value
Age (year)			0.465
≥60	27	−4.04±1.26
<60	8	−4.45±1.75
Gender			0.887
Female	15	−4.18±1.57
Male	20	−4.10±1.24
Diameter (cm)			0.208
≥3	24	−3.93±1.26
<3	11	−4.57±1.56
Differentiation			0.630
Middle–Low	29	−4.19±1.39
High	6	−3.88±1.36
Lymphatic metastasis			0.440
No	7	−3.84±1.00
Yes	28	−4.21±1.45
TNM stage			0.000*
IA–IIB	24	−4.71±1.17
IIIA–IV	11	−2.88±0.84

Note:

* P<0.05.

Figure 2 Relative circ-DLG1 expression and its clinical significance in ESCC plasmas and tissues.

Notes: (A) The expression of circ-DLG1 levels was significantly higher in ESCC plasmas than that in normal plasmas. (B) The expression of circ-DLG1 expression levels was significantly higher in ESCC tissues than that in adjacent noncancerous tissues. (C) The AUC of distinguishing ESCC plasmas from healthy controls was 0.6480. (D) The expression level of circ-DLG1 was significantly associated with TNM stage. ***P<0.0001, *P<0.05.
Abbreviations: AUC, area under the ROC curve; ESCC, esophageal squamous cell carcinoma.

Figure 3 The expression of circ-DLG1 in ESCC cell lines.

Notes: (A) The expression of circ-DLG1 in K140, K70, K150, K180, and T10 cells was higher than that in HET-1 significantly. (B) Knockdown of circP-DLG1 was confirmed via qRT-PCR, demonstrating the effective knockdown in ESCC cells. (C) Knockdown of circ-DLG1 inhibited T10 cell proliferation significantly. (D) Knockdown of circ-DLG1 inhibited K180 cell proliferation significantly. (E) Cloning formation experiment showed that knockdown of circ-DLG1 inhibited K180 and T10 cell proliferation significantly. (F) Cell clone formation ratio after knockdown of circ-DLG1 was lower than that of the control group in K180 and T10 cells. **P<0.001, *P<0.05.
Abbreviations: ESCC, esophageal squamous cell carcinoma; ns, not significant; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; NC, normal control.

Figure 4 Circ-DLG1 could serve as a sponge for multiple miRNAs.

Note: A schematic drawing showing the miRNAs and mRNAs associated with circ-DLG1.