Figures & data
Figure 1 Map of the most recurrent amino acid substitutions in the BCR-ABL1 kinase domain in Ph+ clinical samples.
Abbreviations: IM, imatinib; Ph+, Philadelphia chromosome positive.
![Figure 1 Map of the most recurrent amino acid substitutions in the BCR-ABL1 kinase domain in Ph+ clinical samples.](/cms/asset/e3562f0c-97de-48b3-9576-0969e791d14f/dott_a_177019_f0001_c.jpg)
Figure 2 Cytogenetic (A) and molecular (B) analyses of Ph+ ALL patient’s bone marrow sample.
Abbreviations: ALL, acute lymphoblastic leukemia; FISH, fluorescence in situ hybridization; NTC, non-template control; Ph+, Philadelphia chromosome positive.
![Figure 2 Cytogenetic (A) and molecular (B) analyses of Ph+ ALL patient’s bone marrow sample.](/cms/asset/62560173-974d-4657-b477-5ff7b69de199/dott_a_177019_f0002_c.jpg)
Figure 3 Ph+ ALL patient’s novel BCR-ABL1 point mutation and disease molecular monitoring.
Abbreviations: ALL, acute lymphoblastic leukemia; IM, imatinib; Ph+, Philadelphia chromosome positive; WT, wild-type.
![Figure 3 Ph+ ALL patient’s novel BCR-ABL1 point mutation and disease molecular monitoring.](/cms/asset/d0703535-4dd5-40a0-9b1f-39ef7e981c73/dott_a_177019_f0003_c.jpg)
Figure S1 Alignment of Ph+ ALL patient e1a2 nucleotide sequence (Seq 1) with BCR-ABL1 e1a2 reference sequence from GenBank AF113911.1 (Seq 2).
Abbreviations: ALL, acute lymphoblastic leukemia; Ph+, Philadelphia chromosome positive.
![Figure S1 Alignment of Ph+ ALL patient e1a2 nucleotide sequence (Seq 1) with BCR-ABL1 e1a2 reference sequence from GenBank AF113911.1 (Seq 2).](/cms/asset/89f8b5aa-41f7-4bde-8ce7-6b4c7a8c41f3/dott_a_177019_sf0001_b.jpg)
Figure S2 DISOclust disorder prediction results.
![Figure S2 DISOclust disorder prediction results.](/cms/asset/0f51fc65-40e4-4053-acc7-c83ef9126b7b/dott_a_177019_sf0002_c.jpg)
Table S1 Nested-PCR primers for the amplification of ABL1, BCR-ABL1 transcript identification and analysis of BCR-ABL1 mutational status
Table S2 ABL1 protein alignment between different species demonstrates the conservation of the affected amino acid