E2F transcription factor 8 promotes cell proliferation via CCND1/p21 in esophageal squamous cell carcinoma

Figures & data

Figure 1 E2F8 is upregulated in ESCC.

Notes: (A) Expression pattern of E2F8 in 60 paired ESCC tissues and adjacent normal tissues was detected by qRT-PCR/Western blot. (B) Expression pattern of E2F8 in ESCC cell lines and normal human esophageal epithelial cells (HEEC) was detected by qRT-PCR and Western blot (*P<0.05, **P<0.01, ***P<0.001).
Abbreviations: ESCC, esophageal squamous cell carcinoma; T, tumor tissues; N, adjacent non-tumor tissues; qRT-PCR, quantitative real-time PCR.

Figure 2 E2F8 promotes ESCC cell proliferation and arrests cell cycle at S phase.

Notes: (A) qRT-PCR was used to confirm the transfection efficiency of si-E2F8 in TE-10 and that of pcDNA3.1-E2F8 in EC-1. (B–D) Proliferation effects of downregulated E2F8 in TE-10 and those of upregulated E2F8 in EC-1 compared to control group were detected by CCK-8, colony formation, and EdU assays. (E) Effects of downregulated E2F8 on regulating cell cycle in TE-10 and effects of upregulated E2F8 in EC-1 compared to control group were detected by flow cytometry (*P<0.05, **P<0.01, ***P<0.001).
Abbreviations: ESCC, esophageal squamous cell carcinoma; qRT-PCR, quantitative real-time PCR; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; NC, negative control.

Figure 3 Effects of E2F8 on the expression of CCND1/p21.

Notes: (A, B) Effects of downregulated E2F8 on regulating the expression of CCND1/p21 in TE-10 cells and effects of upregulated E2F8 in EC-1 cells compared to control group were detected by qRT-PCR. (C) Effects of downregulated E2F8 on regulating the expression of CCND1/p21 in TE-10 and effects of upregulated E2F8 in EC-1 compared to control group were detected by Western blot (**P<0.01, ***P<0.001).
Abbreviations: qRT-PCR, quantitative real-time PCR; NC, negative control.

Figure 4 Downregulated E2F8 inhibits tumorigenicity in vivo.

Notes: (A) Tumors generated by TE-10 which were transfected with shE2F8 were compared to tumors in the control group. (B) TE-10 transfected with shE2F8 generated smaller tumors. (C) TE-10 transfected with shE2F8 generated lighter tumors (*P<0.05, **P<0.01).
Abbreviation: NC, negative control.