https://orcid.org/0000-0001-7254-0717
Figures & data
Figure 1 The phenotype of ADSCs and DFs. ADSCs displayed the mesenchymal stem cell phenotype, being negative for CD14 (A), CD34 (B), CD45 (C), HLA-DR (D), and positive for CD44 (E) CD73 (F), CD90 (G) and CD105 (H). They also were differentiated into mesodermal cells including chondrocytes (I), osteoblasts (J) and adipocytes (K). DFs strongly expressed CD90 (L) and vimentin (M).
Abbreviations: ADSCs, adipose-derived stem cells; CD, cluster of differentiation; DFs, dermal fibroblasts.
Table 1 PDLs of ADSCs and DFs at the 3rd, 6th, 9th and 12th Passages
Figure 2 IC50 values of doxorubicin are different between the passages of DFs and ADSCs. The IC50 values of DFs were significantly decreased as the passages of DFs increased, while the values slightly changed when the passage of ADSCs increased from the 3rd to the 6th passage, and from the 6th to the 9th or 12th passage (A). The IC50 values of doxorubicin are nonsignificant between DFs and ADSCs at the 3rd passage; however, these values are significantly different between DFs and ADSCs at the 6th, 9th and 12th passages (B).
Abbreviations: ADSCs, adipose-derived stem cells; DFs, dermal fibroblasts; IC50, the half maximal inhibitory concentration.
Figure 3 The SEI values of doxorubicin on DFs and ADSCs at their different passages when compared to HepG2 and MCF-7 cells. (A) SEI values were significant increased as the passages of DFs increased from the 3rd to 6th, 9th and 12 passages- in the HepG2 model; however, the SEI values changed for ADSCs from the 3rd through 12th passage in that model. (B) Similarly, the SEI values also strongly increased when using DFs as the normal cell control, while the SEI values were nonsignificantly changed when ADSCs were used as the normal cell control in the MCF-7 model.
Abbreviations: ADSCs, adipose-derived stem cells; DFs, dermal fibroblasts; SEI, side effect index.
