Expression and Clinical Value of LncRNA GAPLINC in Esophageal Squamous Cell Carcinoma

Figures & data

Table 1 Relationship Between LncRNA GAPLINC and Clinical Characteristics of ESCC Patients

Clinical Information	No.	%	P value
Gender			P=0.428
Male	34	85
Female	6	15
Age			P=0.269
>60	31	77.5
≤60	9	22.5
Smoking			P=0.152
Yes	16	40
No	24	60
Drinking			P=0.791
Yes	15	37.5
No	25	62.5
Tumor location			vs Low in chest
High in chest	5	12.5	P=0.648
Middle in chest	14	35	P=0.444
Lower middle in chest	10	25	P=0.764
Low in chest	10	25
Unknown	1	2.5
Tumor size			P=0.629
>4cm	20	50
≤4cm	20	50
Differentiation			vs High
High	4	10
Higher middle	6	15	P=0.015
Middle	13	32.5	P=0.011
Lower middle	8	20	P=0.017
Low	9	22.5	P=0.022
Infiltration			vs All
Mucosa and submucosa	5	12.5	P=0.700
Superficial muscularis	2	5	P=0.979
Deep muscularis	5	12.5	P=0.538
All	28	70
Lymph node metastasis
Yes	15	35.5	P=0.114
No	25	62.5

Note: Chi-square test P<0.05.

Figure 1 LncRNA GAPLINC expression levels in ESCC tissues, adjacent normal esophagus tissues, and ESCC cell lines. (A) LncRNA GAPLINC expression levels in ESCC tissues and adjacent normal esophagus tissues. (B) LncRNA GAPLINC expression in five human ESCC cell lines. (*p<0.05).

Figure 2 Receiver operating characteristics (ROC) curves for LncRNA GAPLINC. AUC of LncRNA GAPLINC in the diagnosis of patients was 0.715.

Figure 3 Effects of LncRNA GAPLINC on TE11 cell behavior. (A) LncRNA GAPLINC expression in control, and cells with LncRNA GAPLINC knockdown and overexpression (B) Cell proliferation and (C) cell migration following lncGAPLINC-knockdown and lncGAPLINC-overexpression. (D) Representative images of the cell migration assay. (E) Cell invasion following lncGAPLINC-knockdown and lncGAPLINC-overexpression. (F) Representative images of the invasion assay. (G) Apoptosis following lncGAPLINC-knockdown and lncGAPLINC-overexpression. (*p<0.05).

Table 2 Apoptosis Rate of LncGAPLINC-Knockdown Cell Line and LncGAPLINC-Overexpression Cell Line

	Control	Silence	Overexpression
Apoptosis rate (%)	0.49±0.045	35.97±3.58	0.70±0.083