64
Views
3
CrossRef citations to date
0
Altmetric
Original Research

Oncogenic mutation profiling in new lung cancer and mesothelioma cell lines

, , , , , , , , , , & show all
Pages 195-209 | Published online: 16 Jan 2015

Figures & data

Table 1 A table summarizing the clinical characteristics of patients from which these new cell lines were derived and established

Figure 1 Photomicrographs showing morphological and immunohistochemical properties of different cell lines.

Notes: Side-by-side photomicrographs at 40× and 200× magnification, respectively, for each of FA34 (A and B), FA44 (C and D), FA49 (E and F), FA98 (G and H), and PME1 (I and J) cell lines.
Figure 1 Photomicrographs showing morphological and immunohistochemical properties of different cell lines.

Figure 2 Immunohistochemical staining with TTF1, CK7, and CK20 on paraffin cell blocks for all four lung adenocarcinoma cell lines.

Notes: (AD) TTF1 positive staining for each cell line, (EH) CK7 positive staining for each line, and (IL) CK20 negative staining for each line.
Figure 2 Immunohistochemical staining with TTF1, CK7, and CK20 on paraffin cell blocks for all four lung adenocarcinoma cell lines.
Figure 2 Immunohistochemical staining with TTF1, CK7, and CK20 on paraffin cell blocks for all four lung adenocarcinoma cell lines.

Figure 3 Immunohistochemical staining with Napsin A on paraffin cell blocks for all the four lung adenocarcinoma cell lines.

Notes: (A) FA34, (B) FA44, (C) FA49, and (D) FA98.
Figure 3 Immunohistochemical staining with Napsin A on paraffin cell blocks for all the four lung adenocarcinoma cell lines.

Figure 4 Immunohistochemical staining on paraffin cell blocks from PME1.

Notes: Staining with (A) anti-vimentin a, (B) anti-vimentin b, (C) mucin negative, (D) anti-CK a, (E) anti-CK b, (F) calretinin negative, and (G) WT1 negative.
Figure 4 Immunohistochemical staining on paraffin cell blocks from PME1.

Figure 5 Growth curves of all the cell lines showing the initial lag phase followed by accelerated growth phase in most cell lines with FA44, FA49, and FA98 showing the fastest growth rate compared to FA34 and PME1.

Figure 5 Growth curves of all the cell lines showing the initial lag phase followed by accelerated growth phase in most cell lines with FA44, FA49, and FA98 showing the fastest growth rate compared to FA34 and PME1.

Figure 6 ALK immunohistochemistry and FISH.

Notes: (A) Paraffin cell block section of FA34 showed positive ALK immunohistochemical staining; (B) ALK rearrangement with FISHfor FA34; and (C) FA44 as a control showing no ALK rearrangement with FISH.
Figure 6 ALK immunohistochemistry and FISH.

Figure 7 Spectral karyotyping images of all the cell lines.

Notes: (A) FA34; (B) FA44; (C) FA49; (D) FA98; and (E) PME1.
Figure 7 Spectral karyotyping images of all the cell lines.
Figure 7 Spectral karyotyping images of all the cell lines.

Table 2 Karyotype descriptions in newly established lung cancer cells lines analyzed using spectral karyotyping

Table 3 Summary of mutations of the five cell lines analyzed with targeted sequencing (RainDance OncoSeq)