Hyperthermia combined with 5-fluorouracil promoted apoptosis and enhanced thermotolerance in human gastric cancer cell line SGC-7901

Figures & data

Figure 1 Hyperthermia combined with 5-FU promoted apoptosis and enhanced thermotolerance.

Notes: (A) Survival rates (%) of SGC-7901 cells after thermo-chemotherapy treatments. (B) Inhibition rates (%) of SGC-7901 cells after thermo-chemotherapy treatments. (C) Apoptosis rates (%) of SGC-7901 cells after thermo-chemotherapy treatments. (D) The expression of Bcl-2 and HSP70 protein. *P<0.05 and **P<0.01, a significant difference was observed from the control (40°C). ^#P<0.05, a significant difference was observed from the thermo-chemotherapy group (43°C+5-FU).
Abbreviations: NS, no significance; 5-FU, 5-fluorouracil.

Table 1 Survival rates of SGC-7901 cells after treatments (%)a

Groups	Temperature
	40°C	43°C	46°C
Control	100±0	–	–
Chemotherapy	71.2±3.1	–	–
Thermotherapy	82.9±2.9	79.6±3.0	73.9±2.6
Thermo-chemotherapy	56.3±2.3b,c	45.3±3.5b–d	27.6±3.2b–d

Notes:

a Results were in the form of means ± SD for experiments in triplicate;

b P<0.05 compared with thermotherapy group;

c P<0.05 compared with chemotherapy group;

d P<0.05 compared with thermo-chemotherapy group at 40°C.

Table 2 Inhibition rates of SGC-7901 cells after treatments (%)a

Groups	Temperature
	40°C	43°C	46°C
Control	0	–	–
Chemotherapy	44.2±2.9	–	–
Thermotherapy	27.9±3.6	32.6±3.2	34.2±4.1
Thermo-chemotherapy	52.7±2.0b,c	62.1±3.4b,c	69.5±4.2b,c

Notes:

a Results were in the form of means ± SD for experiments in triplicate;

b P<0.05 compared with thermotherapy group;

c P<0.05 compared with chemotherapy group.

Table 3 Cell apoptosis rates of SGC-7901 cells after treatments (%)a

Groups	Temperature
	40°C	43°C	46°C
Control	0	–	–
Chemotherapy	15.2±1.1	–	–
Thermotherapy	9.1±0.3	12.9±0.5	14.3±1.0
Thermo-chemotherapy	20.3±1.3b,c	31.3±2.5b–d	32.7±2.3b–d

Notes:

a Results were in the form of means ± SD for experiments in triplicate;

b P<0.05 compared with thermotherapy group;

c P<0.05 compared with chemotherapy group;

d P<0.05 compared with thermo-chemotherapy group at 40°C.