Role of NSC319726 in ovarian cancer based on the bioinformatics analyses

Figures & data

Figure 1 Heatmap of differentially expressed genes (DEGs) between samples of human epithelial ovarian cancer cell line TOV112D untreated and treated with NSC319726.

Notes: Red: upregulated DEGs; green: downregulated DEGs. Untreated 1–3: three identical samples of TOV112D cells untreated with NSC319726. Treated 1–3: three identical samples of TOV112D cells treated with NSC319726.

Table 1 KEGG pathways impacted by differentially expressed genes (DEGs) in samples of human epithelial ovarian cancer cell line TOV112D treated with NSC319726

Name	ID	Count	PG	PGFDR	Status
Oocyte meiosis	04114	8	0.0007	0.0410	Activated
Mineral absorption	04978	5	0.0029	0.1052	Inhibited
Cell cycle	04110	7	0.0056	0.1344	Inhibited
Vibrio cholera infection	05110	2	0.0218	0.3536	Activated
Progesterone-mediated oocyte maturation	04914	4	0.0246	0.3536	Inhibited
T-cell receptor signaling pathway	04660	2	0.0434	0.4820	Activated

Notes: ID indicates the serial number of pathway; count indicates the number of enriched DEGs; PG indicates the global pathway significance, P-value; status indicates the perturbation of pathway.

Abbreviations: KEGG, Kyoto Encyclopedia of Genes and Genomes; PGFDR, false discovery rate of PG.

Table 2 The results of functional annotation of differentially expressed genes

	TF	Oncogene	TSG	Other TAG
Upregulated	MXI1, NFIL3, FOXD1, BCL6, RORA, FOSL2, ZNF354A, ZNF10	BCL6, SERTAD2	MXI1, EGLN1, MT1G, BNIP3L, TNFAIP3, FOXO3, KLF10	NAMPT
Downregulated	FOXA1, FOXM1, PBX1	PIN1, AURKA, PBX1, HMMR, PTTG1	ST5, STEAP3, LSAMP, RPS6KA6, BUB1B, EFNA5	TACC3, PLK1, BIRC5

Abbreviations: TF, transcription factor; TSG, tumor suppressor gene; TAG, tumor-associated gene.

Figure 2 The constructed protein–protein interaction network of differentially expressed genes (DEGs).

Notes: Pink: upregulated DEGs; green: downregulated DEGs.

Figure 3 The constructed subnetwork of differentially expressed genes (DEGs).

Notes: Pink: upregulated DEGs; green: downregulated DEGs.

Table 3 Gene Ontology (GO) functional enrichment analysis of differentially expressed genes (DEGs) in the subnetwork (top 4)

Category	Term	Name	Count	P-value
BP	GO:0000278	Mitotic cell cycle	35	0
BP	GO:0000280	Nuclear division	28	0
BP	GO:0006996	Organelle organization	34	0
BP	GO:0007017	Microtubule- based process	20	0
CC	GO:0000922	Spindle pole	12	0
CC	GO:0005819	Spindle	21	0
CC	GO:0015630	Microtubule cytoskeleton	26	0
CC	GO:0030496	Midbody	12	0
MF	GO:0008017	Microtubule binding	9	1.44E–10
MF	GO:0015631	Tubulin binding	9	2.37E–09
MF	GO:0003777	Microtubule motor activity	6	4.39E–08
MF	GO:0005515	Protein binding	35	3.89E–07

Notes: Category stands for the GO functional categories; count stands for the number of enriched DEGs.

Abbreviations: BP, biological process; CC, cellular component; MF, molecular function.