Figures & data
Figure 1 Progestin-resistant cells show increased cell growth and decreased autophagy.
Abbreviations: MTT, 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide; PR, progesterone receptor; PR-A, progesterone receptor isoform A; PR-B, progesterone isoform B; OD, optical density; h, hours.
![Figure 1 Progestin-resistant cells show increased cell growth and decreased autophagy.](/cms/asset/27ae9af7-4739-4480-95e1-6f4c231eb3a3/dott_a_95267_f0001_c.jpg)
Figure 2 Progestin activates the PI3K/AKT/mTOR pathway in progestin-resistant endometrial cancer cells.
Abbreviations: PI3K, phosphatidylinositol 3-kinase; AKT, protein kinase B; mTOR, mechanistic target of rapamycin; MTT, 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide; OD, optical density; Ctrl, control; h, hours; pAKT, phosphor-AKT; pmTOR, phosphor-mTOR; siAKT1, short interfering RNA targeting AKT1; siCtrl, short interfering RNA control with scrambled sequence.
![Figure 2 Progestin activates the PI3K/AKT/mTOR pathway in progestin-resistant endometrial cancer cells.](/cms/asset/96e07fc6-2a7a-4c21-ada3-4459a2324a8e/dott_a_95267_f0002_c.jpg)
Figure 3 Phosphorylation of mTOR is sufficient and necessary for cell growth in endometrial cancer cells by regulating autophagy.
Abbreviations: mTOR, mechanistic target of rapamycin; PR, progesterone receptor; MTT, 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide; OD, optical density; Ctrl, control; h, hours; pmTOR, phosphor-mTOR; siCtrl, short interfering RNA control with scrambled sequence; mut, mutant; simTOR, short interfering RNA targeting mTOR.
![Figure 3 Phosphorylation of mTOR is sufficient and necessary for cell growth in endometrial cancer cells by regulating autophagy.](/cms/asset/88802e39-ba92-4a08-8158-c4af0a3bb818/dott_a_95267_f0003_c.jpg)
Figure 4 RAD001 inhibits proliferation in progestin-resistant endometrial cancer cells by inducing autophagy.
Abbreviations: mTOR, mechanistic target of rapamycin; MTT, 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide; Ctrl, control; OD, optical density; h, hours.
![Figure 4 RAD001 inhibits proliferation in progestin-resistant endometrial cancer cells by inducing autophagy.](/cms/asset/db9e3982-516d-4cca-9f99-55c879ea00f5/dott_a_95267_f0004_c.jpg)