Expression of CD40 is a positive prognostic factor of diffuse large B-cell lymphoma treated with R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone)

Figures & data

Table 1 Summary of clinical characteristics of 186 patients with diffuse large B-cell lymphoma

Characteristics	Number of patients (%)
Age (range), years	(16–85)
Median	64.2
≤64	79 (42.5)
>64	107 (57.5)
Sex
Male	113 (60.7)
Female	73 (39.3)
Performance status
0	33 (17.7)
1	59 (31.7)
2	65 (35)
3	20 (10.8)
4	9 (4.8)
Lactate dehydrogenase
Normal	103 (60.7)
High	83 (39.3)
Extranodal involvement
Yes	105 (56.5)
No	81 (43.5)
Stage
I	23 (12.4)
II	49 (26.3)
III	75 (40.3)
IV	39 (21)
International Prognostic Index
0	8 (4.3)
1	17 (9.1)
2	31 (16.7)
3	54 (29)
4	50 (26.9)
5	26 (14)

Figure 1 CD40 immunohistochemistry in tissues of DLBCL patients.

Notes: (A) Low and (B) high CD40 expression in a case of DLBCL ×200 magnification.
Abbreviation: DLBCL, diffuse large B-cell lymphoma.

Table 2 Association between CD40 expression (IOD/AOI) and different clinicopathological features of diffuse large B-cell lymphoma

Clinicopathological features	Number of cases	CD40 expression	P-value
Age
≤64	79	99.42±6.94
>64	107	93.81±9.35	0.5362
Sex
Male	113	95.33±7.87
Female	73	89.57±6.24	0.6129
Lactate dehydrogenase
Normal	103	96.11±6.49
High	83	88.96±8.42	0.3985
Stage
I	23	101.03±7.13
II	49	92.88±6.99
III	75	95.21±5.78
IV	39	89.79±9.27	0.2408
IPI
Low risk	25	99.58±8.81
Low to intermediate risk	31	94.11±5.99
Intermediate to high risk	54	91.23±7.67
High risk	76	87.98±10.35	0.3647

Notes: Data are presented as Mean ± SD. Results are in response to a one way analysis of variance (ANOVA). P-values were considered significant at <0.05.

Abbreviations: IOD, integrated optical density; AOI, area of interest; SD, standard deviation, IPI, International Prognostic Index.

Figure 2 Mean expression of CD40 using immunohistochemistry in tumors CR, PR, NR, and control.

Notes: *P<0.05. The black lines connecting different bars represent the comparison made between the two. Comparisons were made between CR with PR and CR vs NR, PR vs NR. P-values were considered significant at <0.05.
Abbreviations: CR, complete response; PR, partial response; NR, no response; IOD, integrated optical density; AOI, area of interest.

Figure 3 Overall survival (A) and progression-free survival (B) curves of patients according to CD40 protein expression level.

Note: P<0.05.