Genetic Polymorphism of NQO1 Influences Susceptibility to Coronary Heart Disease in a Chinese Population: A Cross-Sectional Study and Meta-Anaylsis

Figures & data

Table 1 Participates Characteristics of Both CHD Group and Control Group

Characteristics	CHD Group (N=120)	Control Group (N=210)	p
Age	64.8±9.8	63.6±10.2	>0.05
Men	84 (70.0%)	151 (71.9%)	>0.05
BMI	24.8±2.2	23.9±1.6	>0.05
Smokers	26 (21.7%)	43 (20.5%)	>0.05
Diabetes	10 (8.3%)	20 (9.5%)	>0.05
Hypertension	16 (13.3%)	25 (11.9%)	>0.05

Abbreviations: CHD, Coronary heart disease; BMI, body mass index; HDL, high density lipoprotein; LDL, low density lipoprotein.

Table 2 Comparison of Genotype and Allele Frequency Between CHD Group and Control Group

NQO1 C609T	Control Group (N=210)		CHD Group (N=120)		OR (95% CI) ^a	P^a
	n	Percentage (%)	n	Percentage (%)
CC	66	31.4	24	20.0	1.00^REF
CT	64	30.5	36	30.0	1.55 (0.83–2.88)	0.220
TT	80	38.1	60	50.0	2.06 (1.16–3.66)	0.018
C	196	46.7	84	35.0	1.00^REF
T	224	53.3	156	65.0	1.63 (1.17–2.25)	0.005

Note: ^aAdjusted for sex and age by logistic regression model.

Abbreviations: CHD, Coronary heart disease; OR, odds ratio; CI, confidential index.

Table 3 Main Characteristics of All Case-Control Studies Included in Meta-Analysis

Literatures	Ethnics (Country)	Genotyping Methods	Source of Control	Sample Size	HWE Conformity	Genotype Frequency (Case)			Genotype Frequency (Control)			Year
						CC	CT	TT	CC	CT	TT
Boroumand et al^Citation13	Asian (Iran)	PCR-RFLP	HB	143/175	Yes	103	39	1	114	60	1	2015
Yu et al^Citation16	Asian (China)	PCR- RFLP	PB	1142/1106	Yes	346	527	249	324	535	247	2013
Ramprasath et al^Citation15	Asian (India)	PCR-RFLP	HB	298/241	Yes	89	142	67	87	114	40	2012

Abbreviations: PB, Population-based; HB, Hospital-based; HWE, Hardy-Weinberg equilibrium; RFLP, Restricted Fragment Length Polymorphism.

Figure 1 PRISMA 2009 Flow Diagram.

Figure 2 Forest plot for the associations between NQO1 C609T polymorphism and CHD risk through allele contrast (T vs C). Data from these studies.^13,15,16

Abbreviations: OR, odds ratio; CI, confidence interval; CHD, coronary heart disease.

Figure 3 Forest plot for the associations between NQO1 C609T polymorphism and CHD risk through homozygote comparison (TT vs CC). Data from these studies.^13,15,16

Abbreviations: OR, odds ratio; CI, confidence interval; CHD, coronary heart disease.

Figure 4 Forest plot for the associations between NQO1 C609T polymorphism and CHD risk through recessive genetic model (TT vs TC/CC). Data from these studies.^13,15,16

Abbreviations: OR, odds ratio; CI, confidence interval; CHD, coronary heart disease.

Figure 5 Forest plot for the associations between NQO1 C609T polymorphism and CHD risk through dominate genetic model (TT/TC vs CC). Data from these studies.^13,15,16

Abbreviations: OR, odds ratio; CI, confidence interval; CHD, coronary heart disease.

Table 4 Meta-Analysis of the NQO1 C609T Polymorphism and CHD Risk

Comparison	Population	N	Test of Association			Mode	Test of Heterogeneity
			OR	95% CI	P		χ^2	P	I^2
T versus. C	Asian	3	1.02	0.81–1.29	0.910	Random	149.32	0.061	64.3
TT versus. CC	Asian	3	1.17	0.75–1.82	0.623	Random	73.56	0.138	49.4
TT versus. CT/CC	Asian	3	1.11	0.85–1.43	0.788	Random	164.29	0.287	19.8
TT/CT versus. CC	Asian	3	0.98	0.74–1.30	0.623	Random	73.56	0.105	55.6

Abbreviations: CHD, Coronary heart disease; OR, odds ratio; CI, confidence interval.

Boroumand M, Pourgholi L, Goodarzynejad H, et al. NQO1 C609T polymorphism is associated with coronary artery disease in a gender-dependent manner. Cardiovasc Toxicol. 2017;17(1):35–41. doi:10.1007/s12012-015-9353-8

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