To Investigate the Influence of Smoking Cessation Intention and Common Downstream Variants of HDAC9 Gene on Large Artery Atherosclerotic Cerebral Infarction

Figures & data

Table 1 Basic Information of SNPs for Genotyping

Sequence No.	SNP	A Location on a Chromosome	A location on a gene	Major > Minor Allelic Gene	Minor Alleles	MAF Case Health
1	rs10227612	Chr7: 18,994,956	Intron region	G>T	G	0.4173 0.3544
2	rs12669496	Chr7: 19,008,849	3’ end	T>C	T	0.2359 0.2278
3	rs1548577	Chr7: 19,009,539	3’ end	G>A	A	0.6109 0.5443
4	rs2074633	Chr7: 18,996,297	3’ untranslated region	C>T	C	0.3750 0.3059
5	rs2526626	Chr7: 19,001,714	3’ untranslated zone	A>G	A	0.2339 0.2215
6	rs2717344	Chr7: 18,991,230	Intron region	A>G	A	0.2964 0.3629

Abbreviation: MAF stands for minor allele frequency; ^aThe reference genome sequence GRCh38/hg38 was used to determine the chromosomal position.

Table 2 Hardy-Weinberg Equilibrium Test for Genotype Distribution Frequencies of SNPs in the Case Group and the Control Group

Serial No.	Locus	Groups	χ^2	P
1	rs10227612	Case group	0.0973	0.7551
		Control group	4.2104	0.0402
2	rs12669496	Case group	0.0793	0.7782
		Control group	0.3922	0.5311
3	rs1548577	Case group	0.8991	0.3430
		Control group	1.5702	0.2102
4	rs2074633	Case group	0.7168	0.3972
		Control group	4.3554	0.0369
5	rs2526626	Case group	0.3074	0.5793
		Control group	0.2664	0.6058
6	rs2717344	Case group	0.0569	0.8115
		Control group	3.4249	0.0642

Table 3 Comparison of Genotype Frequency and Allele Frequency Between Case Group and Control Group

Sequence No.	Locus	Genotype Alleles	Case Group (n=248) Genotype/Allele Frequency	Control Group (n=237) Genotype/Allele Frequencies	χ^2	P
1	rs10227612	TT	83 (33.47%)	106 (44.73%)	6.745	0.034
		GT	123 (49.60%)	94 (39.66%)
		GG	42 (16.94%)	37 (15.61%)
		T	289 (58.27%)	306 (64.56%)	4.045	0.044
		G	207 (41.73%)	168 (35.44%)
2	rs12669496	CC	144 (58.06%)	143 (60.34%)	0.499	0.779
		CT	91 (36.69%)	80 (33.76%)
		TT	13 (5.24%).	14 (5.91%).
		C	379 (76.41%)	366 (77.22%)	0.088	0.767
		T	117 (23.59%)	108 (22.78%)
3	rs1548577	AA	89 (35.89%)	75 (31.65%)	6.735	0.034
		GA	125 (50.40%)	108 (45.57%)
		GG	34 (13.71%).	54 (22.78%).
		A	303 (61.09%)	258 (54.43%)	6.063	0.014
		G	193 (38.91%)	226 (45.57%)
4	rs2074633	TT	100 (40.32%)	121 (51.05%)	5.643	0.060
		CT	110 (44.35%)	87 (36.71%)
		CC	38 (15.32%).	29 (12.24%).
		T	310 (62.50%)	329 (69.41%)	5.147	0.023
		C	186 (37.50%)	145 (30.59%)
5	rs2526626	GG	144 (58.06%)	145 (61.18%)	0.783	0.676
		GA	92 (37.10%)	79 (33.33%)
		AA	12 (4.84%).	13 (5.49%).
		G	380 (76.61%)	369 (77.85%)	0.210	0.647
		A	116 (23.39%)	105 (22.15%)
6	rs2717344	GG	122 (49.19%)	103 (43.46%)	6.660	0.036
		GA	105 (42.34%)	96 (40.51%)
		AA	21 (8.47%).	38 (16.03%).
		G	349 (70.36%)	302 (63.71%)	4.856	0.028
		A	147 (29.64%)	172 (36.29%)

Table 4 Logistic Regression Analysis of HDAC9 Gene SNP Alleles and LAA-S

Serial No.	Locus	Risk Allele	OR (95% CI)	P
1	rs10227612	T	0.767 (0.591–0.993)	0.044
2	rs12670036	A	0.771 (0.596–0.998)	0.048
3	rs1548577	A	1.375 (1.067–1.773)	0.014
4	rs2074633	T	0.735 (0.563–0.959)	0.023

Table 5 Normality Tests of Smoking in Healthy and Case Groups

	Kolmogorov–Smirnova		Shapiro–Wilk
	Statistics	Sig.	Statistics	Sig.
The healthy and case groups smoked	0.365	0.000	0.633	0.000

Table 6 Normality Tests for Smoking Cessation in Case and Healthy Groups

	Kolmogorov–Smirnova		Shapiro–Wilk
	Statistics	Sig.	Statistics	Sig.
Successful smoking cessation in the case group (3 years of follow-up)	0.389	0.000	0.706	0.000
Successful smoking cessation in the case group (at 7th year of follow-up)	0.387	0.000	0.798	0.000
Successful smoking cessation in the healthy group (3 years of follow-up)	0.388	0.000	0.680	0.000
Successful smoking cessation in the healthy group (at 7 years of follow-up)	0.323	0.000	0.743	0.000

Table 7 Spearman Correlation Coefficient of Smoking Cessation Intention in Case Group

Coefficient of Correlation	Successful Smoking Cessation (at 3 Years of Follow-up)	Successful Smoking Cessation (7 Years of Follow-up)	Intention to Quit Smoking
Successful smoking cessation (3-year follow-up)	1.000	0.518**	0.498**
Successful smoking cessation (at 7 years of follow-up)	0.518**	1.000	0.978**
Willingness to quit smoking	0.498**	0.978**	1.000

Notes: **The correlation is significant at a confidence (two-test) of 0.01.

Table 8 Spearman Correlation Coefficient of Intention to Quit Smoking in Healthy Group

Correlation Coefficient	Successful Smoking Cessation (at 3 Years of Follow-up)	Successful Smoking Cessation (7 Years of Follow-up)	Intention to Quit Smoking
Successful smoking cessation (3-year follow-up)	1.000	0.834**	0.438**
Successful smoking cessation (at 7 years of follow-up)	0.834**	1.000	0.657**
Willingness to quit smoking	0.438**	0.657**	1.000

Notes: **The correlation is significant at a confidence (two-test) of 0.01.