The Effect of Exogenous Superoxide Dismutase (SOD) on Caspase-3 Activation and Apoptosis Induction in Pc-3 Prostate Cancer Cells

Figures & data

Figure 1 Intensity of MnSOD expression on PC-3 cell line.

Table 1 Intensity of MnSOD Expression on PC-3 Cell Line

	Mean Intensity (n=3/group)	P-value
Control negative	13.06±7.21
Control with AB	63.35±1.85	0.0001*
SOD 62.5 mg/mL	152.74±68.14
SOD 83 mg/mL	138.43±17.36
SOD 125 mg/mL	150.02±18.05
SOD 250 mg/mL	154.23±11.46

Note: *One-way ANOVA test.

Table 2 Post Hoc Analysis of MnSOD Expression

Treatment	Comparison Factor	P-value
Control with Antibody	Control negative	0.062
	SOD 62.5 mg/mL	0.004
	SOD 83 mg/mL	0.01
	SOD 125 mg/mL	0.004
	SOD 250 mg/mL	0.003

Figure 2 Caspase 3 expression in PC-3 cell line.

Table 3 Caspase 3 Expression in PC-3 Cell Line

	Mean±SD (%) (n=3/group)	P-value
Control	8.07±1.36	0.016*
SOD 62.5 mg	19.73±0.58
SOD 83 mg	29.13±8.24
SOD 125 mg	33.85±12.26
SOD 250 mg	32.45±10.96

Note: *One-way ANOVA test.

Table 4 Post Hoc Analysis of Caspase 3 Expression

Treatment	Comparison Factor	P-value
Control	SOD 62.5 mg/mL	0.113
	SOD 83 mg/mL	0.011
	SOD 125 mg/mL	0.003
	SOD 250 mg/mL	0.005

Figure 3 Apoptosis index expression in PC-3 cell line.

Table 5 Apoptosis Index Expression in PC-3 Cell Line

	Mean±SD (%) (n=3/group)	P-value
Control	24.2±5.25	0.0001*
SOD 62.5 mg	22.25±0.65
SOD 83 mg	48.67±12.76
SOD 125 mg	85.20±1.08
SOD 250 mg	80.05±19.21

Note: *One-way ANOVA test.

Table 6 Post Hoc Analysis of Apoptosis Index

Treatment	Comparison Factor	P-value
Control	SOD 62.5 mg/mL	0.826
	SOD 83 mg/mL	0.018
	SOD 125 mg/mL	0.0001
	SOD 250 mg/mL	0.0001

Table 7 Correlation Between SOD Extract Dose, MnSOD Expression, Caspase-3 Expression, and Apoptotic Index in a Prostate Cancer Cell Line (PC-3)

Correlation of	Correlation Coefficient (rs)	P
SOD dose with MnSOD expression	0.097	0.764
SOD extract dose with expression of caspase-3 SOD extract dose with apoptotic index Expression of caspase-3 with apoptotic index	0.373 0.679 0.682	0.272 0.015 0.015
MnSOD expression with caspase 3 expression	−0.044	0.891
MnSOD expression with apoptosis index	0.102	0.753

Note: r_s=Pearson correlation coefficient.

Figure 4 Proposed mechanism of induction of PC-3 cell line apoptosis by exogenous SOD via intrinsic pathway.