Figures & data
Figure 1 Nestin expression in undifferentiated NSCs. Quantification of nestin expression from three different rats of each gender. A) Positive nestin immunostaining in male B) and female C) NSCs cultured as neurospheres.
Abbreviation: ns, non significant; NSC, neural stem cell.
Figure 2 Retinoic acid-induced expression of neural fate markers. Male (E–H, M–P, U–X) and female (A–D, I–L, Q–T) NSCs were grown as neurospheres prior to being exposed to retinoic acid 1 mM for 10 days. The identification of the neural phenotypes was then performed by immunofluorescence and confocal microscopy. Myelin basic protein (A, E), βIII-tubulin (B, F), GFAP (C, G), vimentin (I, M), GAP-43 (J, N), CNPase (Q, U), doublecortin (R, V), DAPI (K, O, S, W), merge (D, H, L, P, T, X).
Figure 3 Aromatase differential expression in undifferentiated male and female NSCs. Aromatase expression was visualized on consecutive layers by confocal microscopy on NSCs isolated from the SVZ of three-month-old male (A–J) and female (K–S) rats and grown as neurospheres.
Figure 4 Quantification of aromatase expression in undifferentiated NSCs. NSCs isolated from the SVZ of three-month-old male and female rats and grown as neurospheres were then assessed for the level of expression of aromatase. A) The identification and quantification of aromatase expressed was then performed by western blot chemiluminescence using the NSCs of three different rats of each gender. Aromatase expression was also detected by immunofluorescence and confocal microscopy (B, C). Phase contrast (D, E).
Abbreviation: ns, non significant; NSC, neural stem cell; SVZ, subventricular zone.
